WB (Western Blot)
(15 ug of total protein extract from E. coli was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP (DB-HRP) anti-E. coli RNA Sigma 70 (clone 2G10). Proteins were visualized using chemiluminescence detection.)
Mouse RNA Sigma 70 Monoclonal Antibody
Direct-Blot HRP anti-E. coli RNA Sigma 70 Antibody
Reactivity
E coli, Chlamydia trachomatis, Mycobacterium smegmatis, Staphylococcus aureus, Bordetella pertussis, Rhodobacter sphaeroides, Vibrio parahaemolyticus, Pseudomonas aeruginosa, Agrobacterium tumefaciens, Streptococcus faecalis, Arthrobacter globiformis
Applications
Western Blot
Synonyms
RNA Sigma 70, Antibody; Direct-Blot HRP anti-E. coli RNA Sigma 70 Antibody; RNA pol sigma 70; rpoD; sigma D; RNA Polymerase; anti-RNA Sigma 70 antibody
Host
Mouse
Reactivity
E coli, Chlamydia trachomatis, Mycobacterium smegmatis, Staphylococcus aureus, Bordetella pertussis, Rhodobacter sphaeroides, Vibrio parahaemolyticus, Pseudomonas aeruginosa, Agrobacterium tumefaciens, Streptococcus faecalis, Arthrobacter globiformis
Clonality
Monoclonal
Isotype
Mouse IgG2b
Clone Number
2G10
Form/Format
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-RNA Sigma 70 antibody
WB (Western Blot)
Immunogen
Recombinant protein
Biology Area
Cell Biology , Transcription Factors
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Structure
Sigma 70 (σ70) is a 70 kD transcription factor of E. coli RNA polymerase.
Preparation and Storage
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
WB (Western Blot)
(15 ug of total protein extract from E. coli was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP (DB-HRP) anti-E. coli RNA Sigma 70 (clone 2G10). Proteins were visualized using chemiluminescence detection.)
WB (Western Blot)
(15 ug of total protein extract from E. coli was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP (DB-HRP) anti-E. coli RNA Sigma 70 (clone 2G10). Proteins were visualized using chemiluminescence detection.)
Related Product Information for anti-RNA Sigma 70 antibody
RNA polymerase transcribes DNA to synthesize RNA. Prokaryotes contain a single RNA polymerase- core RNA polymerase. RNA polymerase sigma 70 (also known as RNA pol sigma 70 and rpoD) is a part of a family of sigma factor subunits that are required for specific promoter recognition and initiation. RNA pol sigma 70 is the major housekeeping sigma factor responsible for RNA polymerase recognition out of the majority of promoters necessary to transcribe bacterial genes which are expressed during expansion.
Product Categories/Family for anti-RNA Sigma 70 antibody
References
Breyer MJ, et al. 1997. J. Bacterial 179:1404.
Lesley SA, et al. 1989. J. Biol. Chem. 264:3794.
Strickland MS, et al. 1988. Biochemistry 27:5755.
Lesley SA, et al. 1989. J. Biol. Chem. 264:3794.
Strickland MS, et al. 1988. Biochemistry 27:5755.
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Product Notes
The RNA Sigma 70 (Catalog #AAA78936) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Direct-Blot HRP anti-E. coli RNA Sigma 70 Antibody reacts with E coli, Chlamydia trachomatis, Mycobacterium smegmatis, Staphylococcus aureus, Bordetella pertussis, Rhodobacter sphaeroides, Vibrio parahaemolyticus, Pseudomonas aeruginosa, Agrobacterium tumefaciens, Streptococcus faecalis, Arthrobacter globiformis and may cross-react with other species as described in the data sheet. AAA Biotech's RNA Sigma 70 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the RNA Sigma 70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNA Sigma 70, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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