FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells with RNF20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Rabbit anti-Human, Mouse RNF20 Monoclonal Antibody | anti-RNF20 antibody
RNF20 Antibody
Gene Names
RNF20; BRE1; BRE1A; hBRE1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
ProA affinity purified
Synonyms
RNF20, Antibody; RNF20 Antibody; BRE1 A antibody; BRE1 antibody; BRE1 E3 ubiquitin ligase homolog antibody; BRE1-A antibody; BRE1A antibody; BRE1A_HUMAN antibody; E3 ubiquitin-protein ligase BRE1A antibody; hBRE1 antibody; Homolog of S. cerevisiae BRE1 antibody; RING finger protein 20 antibody; Ring finger protein 20 E3 ubiquitin protein ligase antibody; Rnf20 antibody; anti-RNF20 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
SP00-47
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RNF20 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells with RNF20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of K562 cells with RNF20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
WB (Western Blot)
(Western blot analysis of RNF20 on different lysates using anti-RNF20 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Jurkat)
WB (Western Blot)
(Western blot analysis of RNF20 on different lysates using anti-RNF20 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Jurkat)
Related Product Information for anti-RNF20 antibody
Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF20 (ring finger protein 20), also known as BRE1, BRE1A or hBRE1, is a 975 amino acid nuclear protein that belongs to the BRE1 family. As a component of the RNF20/40 complex, RNF20 functions as an E3 ubiquitin-protein ligase that regulates the monoubiquitination and subsequent degradation of select residues on target proteins, such as Histone H2B. RNF20 is required for transcriptional activation of Hox genes and is most likely recruited by p53 to the MDM2 promoter, thereby acting as a transcriptional co-activator. RNF20 contains one zinc finger domain and exists as a homodimer.
Product Categories/Family for anti-RNF20 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
975
NCBI Official Full Name
E3 ubiquitin-protein ligase BRE1A
NCBI Official Synonym Full Names
ring finger protein 20, E3 ubiquitin protein ligase
NCBI Official Symbol
RNF20
NCBI Official Synonym Symbols
BRE1; BRE1A; hBRE1
NCBI Protein Information
E3 ubiquitin-protein ligase BRE1A; BRE1-A; homolog of S. cerevisiae BRE1; BRE1 E3 ubiquitin ligase homolog
UniProt Protein Name
E3 ubiquitin-protein ligase BRE1A
UniProt Gene Name
RNF20
UniProt Synonym Gene Names
BRE1A; BRE1-A; hBRE1
UniProt Entry Name
BRE1A_HUMAN
Similar Products
RNF20 Antibody
Host: Rabbit
Reactivity: Human, Mouse
Apps: Immunohistochemistry, Western Blot, ELISA
RNF20 Antibody
Host: Rabbit
Reactivity: Human, Mouse
Apps: Immunohistochemistry, Western Blot, ELISA
Product Notes
The RNF20 rnf20 (Catalog #AAA312172) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF20 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RNF20 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RNF20 rnf20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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