IF (Immunofluorescence)
(Immunofluorescence of RNF20 in human liver tissue with RNF20 antibody at 20 μg/ml.)
Rabbit anti-Human, Mouse RNF20 Polyclonal Antibody | anti-RNF20 antibody
RNF20 Antibody
Gene Names
RNF20; BRE1; BRE1A; hBRE1
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
RNF20 Antibody is affinity chromatography purified via peptide column.
Synonyms
RNF20, Antibody; RNF20 Antibody; RNF20; BRE1; BRE1A; hBRE1; E3 ubiquitin-protein ligase BRE1A; RING finger protein 20; BRE1-A; ring finger protein 20, E3 ubiquitin protein ligase; anti-RNF20 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Multiple isoforms of RNF20 are known to exist.
Purity/Purification
RNF20 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
975
Applicable Applications for anti-RNF20 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
RNF20 antibody was raised against a 19 amino acid peptide near the amino terminus of human RNF20.
Buffer
RNF20 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
RNF20 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
IF (Immunofluorescence)
(Immunofluorescence of RNF20 in human liver tissue with RNF20 antibody at 20 μg/ml.)
IF (Immunofluorescence)
(Immunofluorescence of RNF20 in human liver tissue with RNF20 antibody at 20 μg/ml.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of RNF20 in human liver tissue with RNF20 antibody at 2.5 μg/ml.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of RNF20 in human liver tissue with RNF20 antibody at 2.5 μg/ml.)
WB (Western Blot)
(Western blot analysis of RNF20 in human liver tissue lysate with RNF20 antibody at (A) 1 and (B) 2 μg/mL.)
WB (Western Blot)
(Western blot analysis of RNF20 in human liver tissue lysate with RNF20 antibody at (A) 1 and (B) 2 μg/mL.)
Related Product Information for anti-RNF20 antibody
RNF20 Antibody: RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 99; 107 kDa
Observed: 100; 110 kDa
Observed: 100; 110 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase BRE1A
NCBI Official Synonym Full Names
ring finger protein 20, E3 ubiquitin protein ligase
NCBI Official Symbol
RNF20
NCBI Official Synonym Symbols
BRE1; BRE1A; hBRE1
NCBI Protein Information
E3 ubiquitin-protein ligase BRE1A; BRE1-A; homolog of S. cerevisiae BRE1; BRE1 E3 ubiquitin ligase homolog
UniProt Protein Name
E3 ubiquitin-protein ligase BRE1A
UniProt Gene Name
RNF20
UniProt Synonym Gene Names
BRE1A; BRE1-A; hBRE1
UniProt Entry Name
BRE1A_HUMAN
Similar Products
RNF20 Antibody
Host: Rabbit
Reactivity: Human, Mouse
Apps: Immunohistochemistry, Western Blot, ELISA
RNF20 Antibody
Host: Rabbit
Reactivity: Human, Mouse
Apps: Immunohistochemistry, Western Blot, ELISA
Product Notes
The RNF20 rnf20 (Catalog #AAA40766) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF20 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RNF20 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the RNF20 rnf20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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