WB (Western Blot)
(Western Blotusinganti-RT1-DantibodyOX-17(AAA72481) Ratlymphnodelysate(35ugproteininRIPAbuffer)wasresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitIgGversionofOX-17(AAA72481)at2ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedrunningsizeforRT1-Dis30.2kDa.)
RT1-D recombinant antibody
Anti-RT1-D [OX-17], Rabbit IgG, kappa
Reactivity
Rat
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Protein A affinity purified
Synonyms
RT1-D, Recombinant Antibody; Anti-RT1-D [OX-17], Rabbit IgG, kappa; RT1-D; Anti-RT1-D [OX-17]; MHC class II RT1D alpha chain antigen; OX17; MRC OX-17; MRC OX17; RT1-D recombinant antibody
Host
Rabbit
Reactivity
Rat
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
OX-17
Specificity
The OX-17 antigenic determinant is isotypic and on the alpha chain. OX-17 labels B but not T cells from lymph node or thoracic duct lymph but very weakly labels thymocytes and does not label erythrocytes. OX-17 does not cross-react with Rat Ia-A or Mouse Ia-E antigen. The target antigen is expressed on antigen presenting cells, including B cells, macrophages and dendritic cells. RT1-D is a rat MHC class II protein. RT1-D is equivalent to mouse I-E antigen.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for RT1-D recombinant antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IP (Immunoprecipitation), WB (Western Blot)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western Blotusinganti-RT1-DantibodyOX-17(AAA72481) Ratlymphnodelysate(35ugproteininRIPAbuffer)wasresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitIgGversionofOX-17(AAA72481)at2ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedrunningsizeforRT1-Dis30.2kDa.)
WB (Western Blot)
(Western Blotusinganti-RT1-DantibodyOX-17(AAA72481) Ratlymphnodelysate(35ugproteininRIPAbuffer)wasresolvedona10%SDSPAGEgelandblotsprobedwiththechimericrabbitIgGversionofOX-17(AAA72481)at2ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasusedandproteinwasdetectedbychemiluminescence.TheexpectedrunningsizeforRT1-Dis30.2kDa.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-RT1-DantibodyOX-17(AAA72481) Immunofluorescenceanalysisofparaformaldehydefixedratsplenocytespermeabilizedwith0.15%TritonandstainedwiththechimericmouseIgG1versionofOX-17(AAA72481)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72481,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-unknownspecificityantibodyfollowedbyAlexaFluor 488secondaryantibody.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedratsplenocyteswithanti-RT1-DantibodyOX-17(AAA72481) Immunofluorescenceanalysisofparaformaldehydefixedratsplenocytespermeabilizedwith0.15%TritonandstainedwiththechimericmouseIgG1versionofOX-17(AAA72481)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(2ug/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72481,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-unknownspecificityantibodyfollowedbyAlexaFluor 488secondaryantibody.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-RT1-DantibodyOX-17(AAA72481) Ratsplenocyteswerefixedusing2%PFA,permeabilisedusing0.5%TritonandstainedwithunimmunizedrabbitIgGantibody(MOPC-21;isotypecontrol,blackline)ortherabbitIgG-chimericversionofOX-17(AAA72481,blueline)atadilutionof1:100for1hatRT.Afterwashing,boundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometryusingtheanti-RT1-DantibodyOX-17(AAA72481) Ratsplenocyteswerefixedusing2%PFA,permeabilisedusing0.5%TritonandstainedwithunimmunizedrabbitIgGantibody(MOPC-21;isotypecontrol,blackline)ortherabbitIgG-chimericversionofOX-17(AAA72481,blueline)atadilutionof1:100for1hatRT.Afterwashing,boundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
Related Product Information for RT1-D recombinant antibody
OX-17 was generated using BALB/c mice raised against rat spleen glycoproteins depleted of the Ia-A antigen using an OX-6 immunoaffinity column.
References
Fukumoto et al Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol 12:237-43 19826456222 N/A6178598
Note on publication:
Describes the generation and use of OX-17 to identify and purify novel Ia antigens..
Note on publication:
Describes the generation and use of OX-17 to identify and purify novel Ia antigens..
NCBI and Uniprot Product Information
UniProt Accession #
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Product Notes
The RT1-D (Catalog #AAA72481) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RT1-D [OX-17], Rabbit IgG, kappa reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RT1-D can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RT1-D for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RT1-D, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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