Application Data
Mouse RT1.A Monoclonal Antibody | anti-RT1.A antibody
Anti-Rat RT1.A Biotin (Clone OX-18) (mouse IgG1)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
RT1.A, Antibody; Anti-Rat RT1.A Biotin (Clone OX-18) (mouse IgG1); RT1.A Biotin (Clone OX-18) (mouse IgG1); Biotin Anti-Rat RT1.A Monoclonal Antibody; anti-RT1.A antibody
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
OX-18
Specificity
RT1.A (Rat RT1.A)
Form/Format
Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography))
Concentration
Antibody Concentration: 0.1 mg/ml (varies by lot)
Applicable Applications for anti-RT1.A antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Immunogen
rat spleen membrane glycoproteins depleted of Ia-A antigens
Donor
BALB/c spleen
Fusion Partner
X63 Ag8.653
Preparation and Storage
Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Application Data
Application Data
Related Product Information for anti-RT1.A antibody
anti-rat RT1.A monoclonal antibody recognizes a monomorphic determinant of rat Class I MHC antigen (RT1.A) and thus reacts with all rat strains tested including AO(RT1u), DA (RT1a), LEW (RT11) and PVG (RT1c). However, quantitative measurements suggest that only a subfraction of the total Class I molecules are recognized (2). MBS520406 labels all peripheral lymphocytes but only a subfraction of thymocytes. It may be that the weakly labelled cells do in fact express some OX-18 antigenic determinants but there is clearly a major quantitative difference amongst thymocytes. This monoclonal used in tissue sections preferentially labels lymphoid cells in the medulla of the thymus including those cells with the marker phenotype of mature T lymphocytes. (2).
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Product Notes
The RT1.A (Catalog #AAA74190) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RT1.A can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the RT1.A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RT1.A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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