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product-image-AAA36401_ICC8.gif ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-RUVBL1 mAb. [Lot No. 2943C1a-1])

Mouse anti-Human RUVBL1 Monoclonal Antibody | anti-RUVBL1 antibody

Mouse monoclonal antibody Anti-Human RUVBL1

Gene Names
RUVBL1; RVB1; TIH1; ECP54; TIP49; INO80H; NMP238; PONTIN; TIP49A; Pontin52
Reactivity
Human
Synonyms
RUVBL1, Antibody; Mouse monoclonal antibody Anti-Human RUVBL1; Homo sapiens RuvB-like 1 (E. coli) (RUVBL1); RVB1; ECP54;TIP49; NMP238; TIP49A; anti-RUVBL1 antibody
Ordering
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2943C1a
Form/Format
Supplied in PBS (3.0 mM KCI, 1.5 mM KH2PO4. 140 mM NaCI, 8.0Mm Na2HPO4(pH7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3.
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
1785
Immunogen
Recombinant
Preparation
This antibody was purified using protein A column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Preparation and Storage
Store at 2-8°C for 1 year. Recommend storing at -20°C for long term storage. Avoid repeated freezing and thawing cycles.

ICC (Immunocytochemistry)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-RUVBL1 mAb. [Lot No. 2943C1a-1])

product-image-AAA36401_ICC8.gif ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-RUVBL1 mAb. [Lot No. 2943C1a-1])

FCM/FACS (Flow Cytometry)

(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RUVBL1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2943C1a-1])

product-image-AAA36401_FCM10.gif FCM/FACS (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RUVBL1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2943C1a-1])

WB (Western Blot)

(Detection of human RUVBL1 by Western blot.Samples: Whole cell lysate (50 ug) from HT-1080 cells. [Lot No. 2943C1a-1]Predicted molecular weight: 50 kDa)

product-image-AAA36401_WB11.gif WB (Western Blot) (Detection of human RUVBL1 by Western blot.Samples: Whole cell lysate (50 ug) from HT-1080 cells. [Lot No. 2943C1a-1]Predicted molecular weight: 50 kDa)

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

product-image-AAA36401_QC13.gif Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-RUVBL1 monoclonal antibody.)

product-image-AAA36401_QC15.gif Quality Control (Western blot analysis of immunized recombinant protein, using anti-RUVBL1 monoclonal antibody.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens RuvB-like AAA ATPase 1 (RUVBL1), mRNA
NCBI Official Synonym Full Names
RuvB-like AAA ATPase 1
NCBI Official Symbol
RUVBL1
NCBI Official Synonym Symbols
RVB1; TIH1; ECP54; TIP49; INO80H; NMP238; PONTIN; TIP49A; Pontin52
NCBI Protein Information
ruvB-like 1; 49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP-interacting protein; 54 kDa erythrocyte cytosolic protein; ECP-54; INO80 complex subunit H; NMP 238; RuvB (E coli homolog)-like 1; TAP54-alpha; TATA binding protein interacting protein 49 kDa; TIP60-associated protein 54-alpha; nuclear matrix protein 238; pontin 52
UniProt Protein Name
RuvB-like 1
UniProt Gene Name
RUVBL1
UniProt Entry Name
RUVB1_HUMAN

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Product Notes

The RUVBL1 ruvbl1 (Catalog #AAA36401) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human RUVBL1 reacts with Human and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "RUVBL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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