WB (Western Blot)
(S100A8 was immunoprecipitated using:Lane A: 0.5 mg HL-60 Whole Cell Lysate 1 uL anti-S100A8 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-S100A8 rabbit monoclonal antibody, at 1:500 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 11 kDaObserved band size :11 kDa)
Rabbit anti-Human S100A8 Monoclonal Antibody | anti-S100A8 antibody
Recombinant Anti-S100A8 Antibody, Rabbit Monoclonal
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A
Synonyms
S100A8, Antibody; Recombinant Anti-S100A8 Antibody, Rabbit Monoclonal; S100A8 Antibody, Rabbit MAb; S100 calcium binding protein A8; Anti-60B8AG Antibody; Anti-CAGA Antibody; Anti-CFAG Antibody; Anti-CGLA Antibody; Anti-CP-10 Antibody; Anti-L1Ag Antibody; Anti-MA387 Antibody; Anti-MIF Antibody; Anti-MRP-8 Antibody; Anti-MRP8 Antibody; Anti-NIF Antibody; Anti-P8 Antibody; anti-S100A8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
203
Specificity
Human S100A8
Purity/Purification
Protein A
Form/Format
0.2um filtered solution in PBS
Applicable Applications for anti-S100A8 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IP: 1-2uL/mg of lysate
Optimal concentrations/dilutions should be determined by the end user.
IP: 1-2uL/mg of lysate
Optimal concentrations/dilutions should be determined by the end user.
Immunogen
Recombinant Human SI00A8 protein
Preparation
Preparation and Storage
This antibody can be stored at 2°C to 8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C.
Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(S100A8 was immunoprecipitated using:Lane A: 0.5 mg HL-60 Whole Cell Lysate 1 uL anti-S100A8 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-S100A8 rabbit monoclonal antibody, at 1:500 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 11 kDaObserved band size :11 kDa)
WB (Western Blot)
(S100A8 was immunoprecipitated using:Lane A: 0.5 mg HL-60 Whole Cell Lysate 1 uL anti-S100A8 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-S100A8 rabbit monoclonal antibody, at 1:500 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 11 kDaObserved band size :11 kDa)
WB (Western Blot)
(Anti-S100A8 rabbit monoclonal antibody at 1:500 dilutionLane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 11 kDaObserved band size: 11 kDa)
WB (Western Blot)
(Anti-S100A8 rabbit monoclonal antibody at 1:500 dilutionLane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 11 kDaObserved band size: 11 kDa)
Product Categories/Family for anti-S100A8 antibody
References
Passey RJ, et al. (1999) S100A8: emerging functions and regulation. J Leukoc Biol. 66(4): 549-56.Gebhardt C, et al. (2006) S100A8 and S100A9 in inflammation and cancer. Biochem Pharmacol. 72(11): 1622-31.Halayko AJ, et al. (2009) S100A8/A9: a mediator of severe asthma pathogenesis and morbidity? Can J Physiol Pharmacol. 87(10): 743-55.Ghavami S, et al. (2009) S100A8/A9: a Janus-faced molecule in cancer therapy and tumorgenesis. Eur J Pharmacol. 625(1-3): 73-83.Ha YS, et al. (2010) mRNA Expression of S100A8 as a Prognostic Marker for Progression of Non-Muscle-Invasive Bladder Cancer. Korean J Urol. 51(1): 15-20.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein S100-A8
NCBI Official Synonym Full Names
S100 calcium binding protein A8
NCBI Official Symbol
S100A8
NCBI Official Synonym Symbols
P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
NCBI Protein Information
protein S100-A8; MRP-8; S100 calcium-binding protein A8 (calgranulin A); calgranulin-A; calprotectin L1L subunit; cystic fibrosis antigen; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; urinary stone protein band A
UniProt Protein Name
Protein S100-A8
UniProt Gene Name
S100A8
UniProt Synonym Gene Names
CAGA; CFAG; MRP8; CFAG; MRP-8; p8
UniProt Entry Name
S10A8_HUMAN
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Product Notes
The S100A8 s100a8 (Catalog #AAA27743) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-S100A8 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's S100A8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:1000 IP: 1-2uL/mg of lysate Optimal concentrations/dilutions should be determined by the end user. Researchers should empirically determine the suitability of the S100A8 s100a8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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