Mouse anti-Human S100A8 / MRP8 Monoclonal Antibody | anti-S100A8 antibody

Mouse Monoclonal [clone CF-145] (IgG2b) to Human S100A8 / MRP8

Cat. Num. AAA51776

• Gene Names: S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Protein G Purified
• Synonyms: S100A8 / MRP8, Antibody; Mouse Monoclonal [clone CF-145] (IgG2b) to Human S100A8 / MRP8; S100A8, 60B8AG, CFAG, CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, CGLA, L1Ag, NIF, p8, Protein S100-A8, MRP8, Urinary stone protein band A, CP-10, MA387, MRP-8; anti-S100A8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: CF-145
• Specificity: Purified human Calgranulin A
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.02% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-S100A8 antibody: FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
• Immunogen Species: S100A8 / MRP8 antibody was raised against Human
• Immunogen: S100A8 / MRP8 antibody was raised against purified granulocyte antigen of human origin
• Antigen Type: Purified protein
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

NCBI and Uniprot Product Information

• NCBI GI #: 21614544
• NCBI GeneID: 6279
• NCBI Accession #: NP_002955.2
• NCBI GenBank Nucleotide #: NM_002964.4
• UniProt Accession #: P05109; Q5SY70; Q9UC84; Q9UC92; Q9UCJ0; Q9UCM6; A8K5L3; D3DV37
• NCBI Official Full Name: protein S100-A8
• NCBI Official Synonym Full Names: S100 calcium binding protein A8
• NCBI Official Symbol: S100A8
• NCBI Official Synonym Symbols: P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
• NCBI Protein Information: protein S100-A8; MRP-8; calgranulin A; calgranulin-A; cystic fibrosis antigen; calprotectin L1L subunit; urinary stone protein band A; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 calcium-binding protein A8 (calgranulin A)
• UniProt Protein Name: Protein S100-A8
• UniProt Gene Name: S100A8
• UniProt Synonym Gene Names: CAGA; CFAG; MRP8; CFAG; MRP-8; p8
• UniProt Entry Name: S10A8_HUMAN