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product-image-AAA296878_AD8.jpg Application Data (Pepinemab inhibited the tumor growth of CT26 on balb/c mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 76.9% at 50 mpk at D24.)

SEMA4D/CD100 recombinant antibody

Anti-SEMA4D/CD100 Reference Antibody (pepinemab)

Gene Names
SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
Reactivity
Human, Mouse
Applications
Functional Assay, Flow Cytometry, Functional Assay, ELISA
Synonyms
SEMA4D/CD100, Recombinant Antibody; Anti-SEMA4D/CD100 Reference Antibody (pepinemab); SEMA4D/CD100 recombinant antibody
Ordering
Host
CHO Cell
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG4SP
Specificity
SEMA4D/CD100
Form/Format
Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.
Concentration
1mg/ml (varies by lot)
Applicable Applications for SEMA4D/CD100 recombinant antibody
FA (Functional Assay), FCM/FACS (Flow Cytometry), ELISA
Endotoxin
< 0.001EU/ug, determined by LAL method.
Conjugation
Unconjugated
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store at -80 degree C for 2 years under sterile conditions; -20 degree C for 1 year under sterile conditions; Avoid repeated freeze-thaw cycles.

Application Data

(Pepinemab inhibited the tumor growth of CT26 on balb/c mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 76.9% at 50 mpk at D24.)

product-image-AAA296878_AD8.jpg Application Data (Pepinemab inhibited the tumor growth of CT26 on balb/c mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 76.9% at 50 mpk at D24.)

FCM/FACS (Flow Cytometry)

(Human C100 HEK293 cells were stained with Anti-SEMA4D / CD100 Reference Antibody (pepinemab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC231=0.3227 nM)

product-image-AAA296878_FCM10.jpg FCM/FACS (Flow Cytometry) (Human C100 HEK293 cells were stained with Anti-SEMA4D / CD100 Reference Antibody (pepinemab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC231=0.3227 nM)

Application Data

(Immobilized human SEMA4D/CD100, His Tag at 2 ug/mL can bind Anti-SEMA4D / CD100 Reference Antibody (pepinemab), EC50=0.0081ug/mL)

product-image-AAA296878_AD11.jpg Application Data (Immobilized human SEMA4D/CD100, His Tag at 2 ug/mL can bind Anti-SEMA4D / CD100 Reference Antibody (pepinemab), EC50=0.0081ug/mL)

Application Data

(The purity of Anti-SEMA4D / CD100 Reference Antibody (pepinemab)is more than 100% ,determined by SEC-HPLC.)

product-image-AAA296878_AD13.jpg Application Data (The purity of Anti-SEMA4D / CD100 Reference Antibody (pepinemab)is more than 100% ,determined by SEC-HPLC.)

SDS-PAGE

(Anti-SEMA4D / CD100 Reference Antibody (pepinemab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%)

product-image-AAA296878_SDS_PAGE15.jpg SDS-PAGE (Anti-SEMA4D / CD100 Reference Antibody (pepinemab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%)
Product Categories/Family for SEMA4D/CD100 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
96,150 Da
NCBI Official Full Name
semaphorin-4D isoform 1
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
NCBI Official Symbol
SEMA4D
NCBI Official Synonym Symbols
CD100; SEMAJ; coll-4; C9orf164; M-sema-G
NCBI Protein Information
semaphorin-4D; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
UniProt Protein Name
Semaphorin-4D
UniProt Gene Name
SEMA4D
UniProt Synonym Gene Names
C9orf164; CD100; SEMAJ
UniProt Entry Name
SEM4D_HUMAN

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Product Notes

The SEMA4D/CD100 sema4d (Catalog #AAA296878) is a Recombinant Antibody produced from CHO Cell and is intended for research purposes only. The product is available for immediate purchase. The Anti-SEMA4D/CD100 Reference Antibody (pepinemab) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SEMA4D/CD100 can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the SEMA4D/CD100 sema4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEMA4D/CD100, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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