IF (Immunofluorescence)
(ICC/IF analysis of SET7/9 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SET7/9 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human SET 7/9 Monoclonal Antibody | anti-SET 7/9 antibody
SET 7/9 antibody
Gene Names
SETD7; KMT7; SET7; SET9; SET7/9
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
SET 7/9, Antibody; SET 7/9 antibody; SET domain-containing protein 7; SETD7; KIAA1717; KMT7; SET7; SET9; SET7/9 Histone methyltransferase; EC 2.1.1.43; Histone H3-K4 methyltransferase SETD7; H3-K4-HMTase SETD7; SET7/9; Lysine N-methyltransferase 7; Histone-lysine N-methyltransferase SETD7; SET domain-containing protein 7 FLJ21193; H3 K4 HMTase; H4 lysine-4 specific; Histone H3 K4 methyltransferase; Histone H3 lysine 4 specific methyltransferase; Histone H4-K4 methyltransferase; Histone lysine N methyltransferase H3 lysine 4 specific SET7; Histone-lysine N-methyltransferase; Lysine methyltransferase; SET 7; SET 7/9; SET 9; SET D7; SET domain containing protein 7; SET domain-containing protein 8,; anti-SET 7/9 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
s4E5
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
366
Applicable Applications for anti-SET 7/9 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human SET7/9 (1-366aa) purified from E. coli
Clone Note
Anti-human SET7/9 mAb, clone s4E5, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant SET7/9.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of SET7/9 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SET7/9 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of SET7/9 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SET7/9 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The Recombinant human SET7/9 protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SET7/9 transfected 293T cell lysate)
WB (Western Blot)
(The Recombinant human SET7/9 protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SET7/9 transfected 293T cell lysate)
Related Product Information for anti-SET 7/9 antibody
Set7/9 is a histone methyltransferase (HMTase) that transfers methyl group to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. Set7/9, unlike most other SET proteins, is exclusively a mono-methylase.
Product Categories/Family for anti-SET 7/9 antibody
References
Bing X, et al., (2003) Nature 421: 652-56.; Taewoo K, et al., (2003) EMBO. 22: 292-303.; Nishioka K, et al., (2002) Genes Dev. 16: 479-89
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,721 Da
NCBI Official Full Name
histone-lysine N-methyltransferase SETD7 isoform 1
NCBI Official Synonym Full Names
SET domain containing lysine methyltransferase 7
NCBI Official Symbol
SETD7
NCBI Official Synonym Symbols
KMT7; SET7; SET9; SET7/9
NCBI Protein Information
histone-lysine N-methyltransferase SETD7
UniProt Protein Name
Histone-lysine N-methyltransferase SETD7
UniProt Gene Name
SETD7
UniProt Synonym Gene Names
KIAA1717; KMT7; SET7; SET9; H3-K4-HMTase SETD7
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Product Notes
The SET 7/9 setd7 (Catalog #AAA47978) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SET 7/9 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SET 7/9 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SET 7/9 setd7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SET 7/9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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