IF (Immunofluorescence)
((TOP)Negative control of hela cells without PE-conjugated goat anti-mouse lgG (whole molecule).PE-conjugated goat anti-mouse lgG emits red fluorescence. (Bottom)Immunofluorescence analysis of SET07 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule).)
Mouse anti-Human, mouse SET07 Monoclonal Antibody | anti-SETD8 antibody
SET07 Antibody
Gene Names
SETD8; SET8; KMT5A; SET07; PR-Set7
Reactivity
Human, mouse
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
SET07, Antibody; SET07 Antibody; N-lysine methyltransferase SETD8; 211-; H4-K20-HMTase SETD8; Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07; PR-Set7; PR/SET07; SET domain-containing protein 8; SETD8; KMT5A; PRSET7; SET07; SET8; anti-SETD8 antibody
Host
Mouse
Reactivity
Human, mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
43AT551.86
Specificity
This SET07 antibody was raised using purified recombinant GST fusion protein encoding the N-terminal region of human SET07.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.79 (varies by lot)
Sequence Length
352
Applicable Applications for anti-SETD8 antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
((TOP)Negative control of hela cells without PE-conjugated goat anti-mouse lgG (whole molecule).PE-conjugated goat anti-mouse lgG emits red fluorescence. (Bottom)Immunofluorescence analysis of SET07 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule).)
IF (Immunofluorescence)
((TOP)Negative control of hela cells without PE-conjugated goat anti-mouse lgG (whole molecule).PE-conjugated goat anti-mouse lgG emits red fluorescence. (Bottom)Immunofluorescence analysis of SET07 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule).)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with SET07 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with SET07 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysates from 293, 293T cell line (from left to right), using SET07 Antibody(43AT551. 86. 76). 43AT551. 86. 76 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from 293, 293T cell line (from left to right), using SET07 Antibody(43AT551. 86. 76). 43AT551. 86. 76 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
References
An miR-502-binding site single-nucleotide polymorphism in the 3'-untranslated region of the SET8 gene is associated with early age of breast cancer onset. Song F, et al. Clin Cancer Res, 2009 Oct 1. PMID 19789321.
Product specificity and mechanism of protein lysine methyltransferases: insights from the histone lysine methyltransferase SET8. Zhang X, et al. Biochemistry, 2008 Jun 24. PMID 18512960.
Catalytic function of the PR-Set7 histone H4 lysine 20 monomethyltransferase is essential for mitotic entry and genomic stability. Houston SI, et al. J Biol Chem, 2008 Jul 11. PMID 18480059.
PR-Set7 establishes a repressive trans-tail histone code that regulates differentiation. Sims JK, et al. Mol Cell Biol, 2008 Jul. PMID 18474616.
SET8 plays a role in controlling G1/S transition by blocking lysine acetylation in histone through binding to H4 N-terminal tail. Yin Y, et al. Cell Cycle, 2008 May 15. PMID 18418072.
Product specificity and mechanism of protein lysine methyltransferases: insights from the histone lysine methyltransferase SET8. Zhang X, et al. Biochemistry, 2008 Jun 24. PMID 18512960.
Catalytic function of the PR-Set7 histone H4 lysine 20 monomethyltransferase is essential for mitotic entry and genomic stability. Houston SI, et al. J Biol Chem, 2008 Jul 11. PMID 18480059.
PR-Set7 establishes a repressive trans-tail histone code that regulates differentiation. Sims JK, et al. Mol Cell Biol, 2008 Jul. PMID 18474616.
SET8 plays a role in controlling G1/S transition by blocking lysine acetylation in histone through binding to H4 N-terminal tail. Yin Y, et al. Cell Cycle, 2008 May 15. PMID 18418072.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42890
NCBI Official Full Name
N-lysine methyltransferase SETD8
NCBI Official Synonym Full Names
SET domain containing (lysine methyltransferase) 8
NCBI Official Symbol
SETD8
NCBI Official Synonym Symbols
SET8; KMT5A; SET07; PR-Set7
NCBI Protein Information
N-lysine methyltransferase SETD8
UniProt Protein Name
N-lysine methyltransferase SETD8
UniProt Gene Name
SETD8
UniProt Synonym Gene Names
KMT5A; PRSET7; SET07; SET8; PR-Set7; PR/SET07
UniProt Entry Name
SETD8_HUMAN
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Product Notes
The SETD8 setd8 (Catalog #AAA283438) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SET07 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SET07 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the SETD8 setd8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SET07, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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