Application Data
(Recombinant SETD8 protein activity assay. Recombinant SETD8 activity titration was assessed using a Radiometry assay. Titration curves were generated to show signal response in the presence of histone peptide substrate at increasing SETD8 concentrations. This data was generated and kindly provided courtesy of ChemPartner.)
SETD8 recombinant protein
Recombinant SETD8 protein
Gene Names
SETD8; SET8; KMT5A; SET07; PR-Set7
Purity
The recombinant protein is >85% pure by SDS-PAGE.
Synonyms
SETD8; N/A; Recombinant SETD8 protein; SET8; KMT5A; SET07; PR-Set7; SETD8 recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >85% pure by SDS-PAGE.
Form/Format
Full length recombinant SETD8 protein is supplied in 25mM Tris pH8.0, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100 and 0.2mg/ml 3× DYKDDDDK peptide.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant SETD8 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant SETD8 protein activity assay. Recombinant SETD8 activity titration was assessed using a Radiometry assay. Titration curves were generated to show signal response in the presence of histone peptide substrate at increasing SETD8 concentrations. This data was generated and kindly provided courtesy of ChemPartner.)
Application Data
(Recombinant SETD8 protein activity assay. Recombinant SETD8 activity titration was assessed using a Radiometry assay. Titration curves were generated to show signal response in the presence of histone peptide substrate at increasing SETD8 concentrations. This data was generated and kindly provided courtesy of ChemPartner.)
SDS-PAGE
(Recombinant SETD8 protein gel. Recombinant SETD8 was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
SDS-PAGE
(Recombinant SETD8 protein gel. Recombinant SETD8 was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
Related Product Information for SETD8 recombinant protein
Short Description: Full-length recombinant SETD8 protein (accession number NP_065115.3) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK-Tag with a molecular weight of 42.2 kDa. The recombinant protein is >85% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: SETD8 (SET Domain Containing (Lysine Methyltransferase) 8) is Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins.It specifically catalyzes monomethylation of histone H4 lysine 20 (H4K20) which is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. H4K20me0 is also involved in DNA damage response. Besides H4K20, SETD8 monomethylates non-histone substrates including proliferating cell nuclear antigen (PCNA) and promotes carcinogenesis by deregulating PCNA expression. It also mediates monomethylation of p53/TP53 at 'Lys-382'and leads to repress p53/TP53-target genes.
Background: SETD8 (SET Domain Containing (Lysine Methyltransferase) 8) is Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins.It specifically catalyzes monomethylation of histone H4 lysine 20 (H4K20) which is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. H4K20me0 is also involved in DNA damage response. Besides H4K20, SETD8 monomethylates non-histone substrates including proliferating cell nuclear antigen (PCNA) and promotes carcinogenesis by deregulating PCNA expression. It also mediates monomethylation of p53/TP53 at 'Lys-382'and leads to repress p53/TP53-target genes.
Product Categories/Family for SETD8 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,223 Da
NCBI Official Full Name
N-lysine methyltransferase SETD8
NCBI Official Synonym Full Names
SET domain containing (lysine methyltransferase) 8
NCBI Official Symbol
SETD8
NCBI Official Synonym Symbols
SET8; KMT5A; SET07; PR-Set7
NCBI Protein Information
N-lysine methyltransferase SETD8; PR/SET07; H4-K20-HMTase SETD8; lysine N-methyltransferase 5A; SET domain-containing protein 8; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; histone-lysine N-methyltransferase SETD8; H4-K20-spec
UniProt Protein Name
N-lysine methyltransferase SETD8
UniProt Gene Name
SETD8
UniProt Synonym Gene Names
KMT5A; PRSET7; SET07; SET8; PR-Set7; PR/SET07
UniProt Entry Name
SETD8_HUMAN
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Product Notes
The SETD8 setd8 (Catalog #AAA59677) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SETD8, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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