IP (Immunoprecipitation)
(Immunoprecipitation of SHC1 from HepG2 whole cell lysate. The Input lane is 10% input; SHC1 p66-IP lane is the immunoprecipitation with the Mouse anti Human SHC1 p66 antibody (2.5 ug, AAA50443); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 ug). Western blot analysis was performed using Mouse anti SHC1 p66 antibody (1/500, AAA50443) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000) and visualized on the ChemiDoc MP. Arrow points to SHC1 p66 isoform.)
Mouse anti-Human SHC1 p66 Monoclonal Antibody | anti-SHC1 antibody
SHC1 p66 antibody | K02/12E8
Gene Names
SHC1; SHC; SHCA
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
SHC1 p66, Antibody; SHC1 p66 antibody | K02/12E8; MOUSE ANTI HUMAN SHC1 p66; anti-SHC1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
K02/12E8
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Purified IgG - liquid
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-SHC1 antibody
WB (Western Blot), IP (Immunoprecipitation)
Species Cross-Reactivity
Mouse: Yes; Rat: Yes
Immunogen
E Coli-derived recombinant protein, aa 1-110 of human SHC1 p66
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
IP (Immunoprecipitation)
(Immunoprecipitation of SHC1 from HepG2 whole cell lysate. The Input lane is 10% input; SHC1 p66-IP lane is the immunoprecipitation with the Mouse anti Human SHC1 p66 antibody (2.5 ug, AAA50443); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 ug). Western blot analysis was performed using Mouse anti SHC1 p66 antibody (1/500, AAA50443) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000) and visualized on the ChemiDoc MP. Arrow points to SHC1 p66 isoform.)
IP (Immunoprecipitation)
(Immunoprecipitation of SHC1 from HepG2 whole cell lysate. The Input lane is 10% input; SHC1 p66-IP lane is the immunoprecipitation with the Mouse anti Human SHC1 p66 antibody (2.5 ug, AAA50443); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 ug). Western blot analysis was performed using Mouse anti SHC1 p66 antibody (1/500, AAA50443) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000) and visualized on the ChemiDoc MP. Arrow points to SHC1 p66 isoform.)
WB (Western Blot)
(Western blot analysis of whole cell lysates probed with SHC1 p66 antibody (2 ug/ml, AAA50443) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000) and visualized on the ChemiDoc MP. Arrow points to SHC1 p66 isoform (molecular weight of 67 kDa).)
WB (Western Blot)
(Western blot analysis of whole cell lysates probed with SHC1 p66 antibody (2 ug/ml, AAA50443) followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000) and visualized on the ChemiDoc MP. Arrow points to SHC1 p66 isoform (molecular weight of 67 kDa).)
WB (Western Blot)
(Western blot analysis of whole cell lysates probed with SHC1 p66 antibody (1 ug/ml, AAA50443) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP. Arrow points to SHC1 p66 isoform (molecular weight of 67 kDa).)
WB (Western Blot)
(Western blot analysis of whole cell lysates probed with SHC1 p66 antibody (1 ug/ml, AAA50443) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP. Arrow points to SHC1 p66 isoform (molecular weight of 67 kDa).)
Related Product Information for anti-SHC1 antibody
Mouse anti Human SHC1 p66 antibody recognizes SRC homology 2 domain-containing transforming protein C1 isoform p66, also known as SHC-transforming protein 3 isoform p66 or SHC-transforming protein A isoform p66.
SHC1 is a ubiquitously-expressed signaling adapter, linking activated growth factor receptors to signaling pathways. Importantly, EGFR signaling is mediated by phosphorylation and interactions of SHC1 protein, defining output from the EGF R pathway (Zheng et al. 2013). SHC1 exists as three functionally-distinct isoforms with molecular weights 46 kDa, 52 kDa and 66 kDa. These isoforms are encoded from the same gene, sharing a common structure and each possessing several phosphorylation sites (Mehlitz et al. 2010). SHC1 plays an oncogenic role in several cancer types (An et al. 2016). Isoforms of SHC1 may have distinct roles in disease.
SHC1 is a ubiquitously-expressed signaling adapter, linking activated growth factor receptors to signaling pathways. Importantly, EGFR signaling is mediated by phosphorylation and interactions of SHC1 protein, defining output from the EGF R pathway (Zheng et al. 2013). SHC1 exists as three functionally-distinct isoforms with molecular weights 46 kDa, 52 kDa and 66 kDa. These isoforms are encoded from the same gene, sharing a common structure and each possessing several phosphorylation sites (Mehlitz et al. 2010). SHC1 plays an oncogenic role in several cancer types (An et al. 2016). Isoforms of SHC1 may have distinct roles in disease.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62,822 Da
NCBI Official Full Name
SHC-transforming protein 1 isoform 3
NCBI Official Synonym Full Names
SHC (Src homology 2 domain containing) transforming protein 1
NCBI Official Symbol
SHC1
NCBI Official Synonym Symbols
SHC; SHCA
NCBI Protein Information
SHC-transforming protein 1; SH2 domain protein C1; SHC-transforming protein 3; SHC-transforming protein A; SHC (Src homology 2 domain-containing) transforming protein 1
UniProt Protein Name
SHC-transforming protein 1
UniProt Gene Name
SHC1
UniProt Synonym Gene Names
SHC; SHCA; SH2 domain protein C1
UniProt Entry Name
SHC1_HUMAN
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Product Notes
The SHC1 shc1 (Catalog #AAA50443) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SHC1 p66 antibody | K02/12E8 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SHC1 p66 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the SHC1 shc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHC1 p66, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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