FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing MCF-7 cells stained with AAA279071 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
SLC1A2 recombinant antibody
SLC1A2 Recombinant Monoclonal Antibody
Gene Names
SLC1A2; EAAT2; GLT-1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Affinity-chromatography
Synonyms
SLC1A2, Recombinant Antibody; SLC1A2 Recombinant Monoclonal Antibody; Excitatory amino acid transporter 2 (Glutamate/aspartate transporter II)(Sodium-dependent glutamate/aspartate transporter 2)(Solute carrier family 1 member 2); SLC1A2; EAAT2 GLT1; SLC1A2 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
28F9
Purity/Purification
Affinity-chromatography
Form/Format
Liquid; PBS, 150mM NaCl, 0.02% sodium azide, 50% glycerol, pH 7.4.
Applicable Applications for SLC1A2 recombinant antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
A synthesized peptide derived from Human SLC1A2
Conjugation
Non-conjugated
Research Area
Neuroscience; Metabolism; Signal transduction
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing MCF-7 cells stained with AAA279071 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing MCF-7 cells stained with AAA279071 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
WB (Western Blot)
(Western BlotPositive WB detected in: U251 whole cell lysateAll lanes: EAAT2 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62 kDaObserved band size: 62 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: U251 whole cell lysateAll lanes: EAAT2 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62 kDaObserved band size: 62 kDa)
Related Product Information for SLC1A2 recombinant antibody
The SLC1A2 recombinant monoclonal antibody is synthesized in vitro through a systematic process. Initially, SLC1A2 antibody genes are isolated from B cells derived from immunoreactive rabbits. These genes undergo amplification and are cloned into phage vectors, which are subsequently introduced into mammalian cell lines to facilitate the generation of functional antibodies in significant quantities. The resulting SLC1A2 recombinant monoclonal antibody is purified from the culture supernatant of the transfected cell lines through affinity chromatography. It can recognize human SLC1A2 protein in three applications, including ELISA, WB, and FC.
SLC1A2, or EAAT2/GLT-1, is a crucial protein responsible for clearing excess glutamate from the synaptic cleft, preventing excitotoxicity, and maintaining proper neurotransmission and brain function. Its role in glutamate transport is fundamental to neuronal health, synaptic plasticity, and the prevention of neurological disorders associated with glutamate dysregulation.
SLC1A2, or EAAT2/GLT-1, is a crucial protein responsible for clearing excess glutamate from the synaptic cleft, preventing excitotoxicity, and maintaining proper neurotransmission and brain function. Its role in glutamate transport is fundamental to neuronal health, synaptic plasticity, and the prevention of neurological disorders associated with glutamate dysregulation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
excitatory amino acid transporter 2 isoform 2
NCBI Official Synonym Full Names
solute carrier family 1 (glial high affinity glutamate transporter), member 2
NCBI Official Symbol
SLC1A2
NCBI Official Synonym Symbols
EAAT2; GLT-1
NCBI Protein Information
excitatory amino acid transporter 2; glutamate/aspartate transporter II; excitotoxic amino acid transporter 2; sodium-dependent glutamate/aspartate transporter 2
UniProt Protein Name
Excitatory amino acid transporter 2
UniProt Gene Name
SLC1A2
UniProt Synonym Gene Names
EAAT2; GLT1
UniProt Entry Name
EAA2_HUMAN
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Product Notes
The SLC1A2 slc1a2 (Catalog #AAA279071) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC1A2 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLC1A2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SLC1A2 slc1a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC1A2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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