FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing A549 cells stained with AAA279327 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
SLC1A5 recombinant antibody
SLC1A5 Recombinant Monoclonal Antibody
Gene Names
SLC1A5; R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
Purity
Affinity-chromatography
Synonyms
SLC1A5, Recombinant Antibody; SLC1A5 Recombinant Monoclonal Antibody; ASCT2 antibody; AAAT antibody; AAAT_HUMAN antibody; ATB(0) antibody; ATBO antibody; Baboon M7 virus receptor antibody; FLJ31068 antibody; M7V1 antibody; M7VS1 antibody; Neutral amino acid transporter B(0) antibody; R16 antibody; RD114/simian type D retrovirus receptor antibody; RDR antibody; RDRC antibody; SLC1A5 antibody; Sodium dependent neutral amino acid transporter type 2 antibody; Sodium-dependent neutral amino acid transporter type 2 antibody; Solute carrier family 1 (neutral amino acid transporter); member 5 antibody; Solute carrier family 1 member 5 antibody$; SLC1A5 recombinant antibody
Host
HEK293F Cell
Reactivity
Human
Clonality
Monoclonal
Isotype
hIgG1
Clone Number
4G12
Purity/Purification
Affinity-chromatography
Form/Format
Liquid; Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Applicable Applications for SLC1A5 recombinant antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA
Antigen Species
Human
Antigen
Recombinant Human SLC1A5 protein
Conjugation
Non-conjugated
Research Area
Cancer, Metabolism, Microbiology, Signal transduction
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing A549 cells stained with AAA279327 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing A549 cells stained with AAA279327 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with AAA279327 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with AAA279327 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF cell with AAA279327 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF cell with AAA279327 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,710 Da
NCBI Official Full Name
neutral amino acid transporter B(0) isoform 1
NCBI Official Synonym Full Names
solute carrier family 1 (neutral amino acid transporter), member 5
NCBI Official Symbol
SLC1A5
NCBI Official Synonym Symbols
R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
NCBI Protein Information
neutral amino acid transporter B(0); neutral amino acid transporter B(0); ATB(0); RD114 virus receptor; baboon M7 virus receptor; solute carrier family 1 member 5; RD114/simian type D retrovirus receptor; sodium-dependent neutral amino acid transporter ty
UniProt Protein Name
Neutral amino acid transporter B(0)
UniProt Gene Name
SLC1A5
UniProt Synonym Gene Names
ASCT2; M7V1; RDR; RDRC; ATB(0)
UniProt Entry Name
AAAT_HUMAN
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Product Notes
The SLC1A5 slc1a5 (Catalog #AAA279327) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The SLC1A5 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLC1A5 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the SLC1A5 slc1a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC1A5, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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