IF (Immunofluorescence)
(ICC/IF analysis of SMAC in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SMAC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human SMAC Monoclonal Antibody | anti-SMAC antibody
SMAC antibody
Gene Names
DIABLO; SMAC; DFNA64
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
SMAC, Antibody; SMAC antibody; Diablo isoform 1; SMAC3; DIABLO-S; FLJ10537; FLJ25049; SMAC/Diablo; Diablo isoform 1 0610041G12Rik; DBOH; Diablo homolog; DIABLO S; Diablo homolog (Drosophila); Mitochondrial Smac protein; Diablo homolog mitochondrial; SMAC 3; Smac protein; Direct IAP binding protein with low pI; Second mitochondria derived activator of caspase.; anti-SMAC antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT19F2
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
239
Applicable Applications for anti-SMAC antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human SMAC/Diablo (56-239aa) purified from E. coli
Clone Note
Anti-human SMAC mAb, clone AT19F2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SMAC/Diablo protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of SMAC in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SMAC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of SMAC in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SMAC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The cell lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: Hep3B cell lysate Lane 4.: MCF7 cell lysate)
WB (Western Blot)
(The cell lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: Hep3B cell lysate Lane 4.: MCF7 cell lysate)
Related Product Information for anti-SMAC antibody
SMAC/Diablo is one of the proapoptotic proteins. SMAC promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding to inhibitor of apoptosis proteins (IAPs) and removing their inhibitory activity. In healthy cells, SMAC is a mitochondrial protein, but when cells undergo apoptosis, SMAC is released into the cytosol. SMAC is ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes
Product Categories/Family for anti-SMAC antibody
References
McNeish. I.A., et al. (2003) Exp Cell Res 286 (2): 186-198; Yu. J., et al. (2007) Oncogene 26 (29): 4189-4198 ; Vucic. D., et al. (2002) J Biol Chem 277 (14): 12275-12279
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,284 Da
NCBI Official Full Name
diablo homolog, mitochondrial isoform 1
NCBI Official Synonym Full Names
diablo IAP-binding mitochondrial protein
NCBI Official Symbol
DIABLO
NCBI Official Synonym Symbols
SMAC; DFNA64
NCBI Protein Information
diablo homolog, mitochondrial
UniProt Protein Name
Diablo homolog, mitochondrial
UniProt Gene Name
DIABLO
UniProt Synonym Gene Names
SMAC; Smac
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Product Notes
The SMAC diablo (Catalog #AAA48126) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SMAC antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMAC can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SMAC diablo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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