SMAC Recombinant Protein | DIABLO recombinant protein

SMAC Protein, Human, Recombinant (His Tag)

Cat. Num. AAA257411

• Gene Names: DIABLO; SMAC; SMAC3; DFNA64; DIABLO-S
• Purity: >90 % as determined by SDS-PAGE
• Synonyms: SMAC; N/A; SMAC Protein, Human, Recombinant (His Tag); Human SMAC/Diablo Protein (His Tag); diablo, IAP-binding mitochondrial protein; DFNA64 Protein, Human; SMAC Protein, Human; DIABLO recombinant protein

• Host: E Coli
• Purity/Purification: >90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 191aa
• Sequence: Ala56-Asp239

• Species: Human
• Tag: C-His
• Predicted N Terminal: Met
• Protein Construction: |A DNA sequence encoding the mitochondrial-located form of human Diablo (NP_063940.1) (Ala 56-Asp 239) was expressed with a polyhistidine tag at the C-terminus
• Activity: Measured by its binding ability in a functional ELISA.Immobilized recombinant human SMAC-His (Cat:10339-H08E)at 10ug/ml (100ul/well) can bind recombinant human XIAP-AVI (Cat:10606-H17E) with a linear range of 0.125-1.0ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized recombinant human SMAC-His (Cat: 10339-H08E)at 10 ug/ml (100 ul/well) can bind recombinant human XIAP-AVI (Cat: 10606-H17E) with a linear range of 0.125-1.0 ug/ml.)

Application Data

Related Product Information for DIABLO recombinant protein

Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer.

Product Categories/Family for DIABLO recombinant protein

Death Receptor Signaling

References

Korga A, et al. (2006) Role of mitochondrial protein Smac/Diablo in regulation of apoptotic pathways Pol Merkur Lekarski. 20(119): 573-6.Anguiano-Hernandez YM, et al. (2007) Smac/DIABLO and colon cancer. Anticancer Agents Med Chem. 7(4): 467-73.Martinez-Ruiz G, et al. (2008) Role of Smac/DIABLO in cancer progression. J Exp Clin Cancer Res. 27: 48.

NCBI and Uniprot Product Information

• NCBI GI #: 9845297
• NCBI GeneID: 56616
• NCBI Accession #: NP_063940.1
• NCBI GenBank Nucleotide #: NM_019887.4
• UniProt Accession #: Q9NR28; Q6W3F3; Q96LV0; Q9BT11; Q9HAV6; B2RDQ0
• Molecular Weight: 27,131 Da
• NCBI Official Full Name: diablo homolog, mitochondrial isoform 1
• NCBI Official Synonym Full Names: diablo, IAP-binding mitochondrial protein
• NCBI Official Symbol: DIABLO
• NCBI Official Synonym Symbols: SMAC; SMAC3; DFNA64; DIABLO-S
• NCBI Protein Information: diablo homolog, mitochondrial; 0610041G12Rik; mitochondrial Smac protein; direct IAP-binding protein with low pI; second mitochondria-derived activator of caspase
• UniProt Protein Name: Diablo homolog, mitochondrial
• UniProt Gene Name: DIABLO
• UniProt Synonym Gene Names: SMAC; Smac
• UniProt Entry Name: DBLOH_HUMAN