WB (Western Blot)
(Detection of human SMAD3 by western blot. Samples: Whole cell lysate (50 ug) from PANC-1, HeLa, A-549, Jurkat, HEK293T, K-562, U2OS, and HT-29 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD3 recombinant monoclonal antibody (AAA213605 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
SMAD3 recombinant antibody
Rabbit anti-SMAD3 Recombinant Monoclonal Antibody [BLR144J]
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
SMAD3, Recombinant Antibody; Rabbit anti-SMAD3 Recombinant Monoclonal Antibody [BLR144J]; mad homolog JV15-2; mad3; SMAD, mothers against DPP homolog 3; SMAD family member 3; SMAD 3; SMA- and MAD-related protein 3; mothers against DPP homolog 3; mothers against decapentaplegic homolog 3; MADH3; Smad3; HsT17436; hMAD-3; MAD, mothers against decapentaplegic homolog 3; HSPC193; JV15-2; LDS1C; LDS3; MAD homolog 3; mad protein homolog; hSMAD3; Mothers against decapentaplegic homolog 3; SMAD3 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR144J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
400ug/ml (varies by lot)
Applicable Applications for SMAD3 recombinant antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 200 and 250
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SMAD3 by western blot. Samples: Whole cell lysate (50 ug) from PANC-1, HeLa, A-549, Jurkat, HEK293T, K-562, U2OS, and HT-29 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD3 recombinant monoclonal antibody (AAA213605 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human SMAD3 by western blot. Samples: Whole cell lysate (50 ug) from PANC-1, HeLa, A-549, Jurkat, HEK293T, K-562, U2OS, and HT-29 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD3 recombinant monoclonal antibody (AAA213605 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human SMAD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from PANC-1 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMAD3 recombinant monoclonal antibody (AAA213605 lot 1) used for IP at 20 ul/mg lysate. SMAD3 was also immunoprecipitated by rabbit anti-SMAD3 antibody BL-1699B-2D12. For blotting immunoprecipitated SMAD3, AAA213605 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human SMAD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from PANC-1 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMAD3 recombinant monoclonal antibody (AAA213605 lot 1) used for IP at 20 ul/mg lysate. SMAD3 was also immunoprecipitated by rabbit anti-SMAD3 antibody BL-1699B-2D12. For blotting immunoprecipitated SMAD3, AAA213605 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for SMAD3 recombinant antibody
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. It also functions as a tumor suppressor. [taken from NCBI Entrez Gene (Gene ID: 4088)].
Product Categories/Family for SMAD3 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,722 Da
NCBI Official Full Name
mothers against decapentaplegic homolog 3 isoform 1
NCBI Official Synonym Full Names
SMAD family member 3
NCBI Official Symbol
SMAD3
NCBI Official Synonym Symbols
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
NCBI Protein Information
mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentapleg
UniProt Protein Name
Mothers against decapentaplegic homolog 3
UniProt Gene Name
SMAD3
UniProt Synonym Gene Names
MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
UniProt Entry Name
SMAD3_HUMAN
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Product Notes
The SMAD3 smad3 (Catalog #AAA213605) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SMAD3 Recombinant Monoclonal Antibody [BLR144J] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMAD3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SMAD3 smad3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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