SDS-PAGE
(SDS-PAGE Analysis of Purified SM-MHC Monoclonal Antibody (SMMS-1). Confirmation of Integrity and Purity of Antibody)
Mouse Smooth Muscle Myosin Heavy Chain (SM-MHC) Monoclonal Antibody | anti-MYH11 antibody
Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Mouse Monoclonal Antibody
Gene Names
MYH11; AAT4; FAA4; SMHC; SMMHC
Reactivity
Cat, Chicken, Cow, Dog, Guinea Pig, Human, Pig, Rabbit, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Synonyms
Smooth Muscle Myosin Heavy Chain (SM-MHC), Antibody; Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Mouse Monoclonal Antibody; AAT4; Myosin heavy chain 11 (MYH11); Myosin heavy chain 11 smooth muscle; Smooth muscle myosin heavy chain 11; Myosin-11; SM1; SM2; SMHC; SMMHC; anti-MYH11 antibody
Host
Mouse
Reactivity
Cat, Chicken, Cow, Dog, Guinea Pig, Human, Pig, Rabbit, Rat
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SMMS-1
Specificity
Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
1945
Applicable Applications for anti-MYH11 antibody
Flow Cytometry (FC), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Cellular Localization
Melanosome
Immunogen
Human uterus extract
Research Areas
Autophagy, Cancer, Cardiovascular, Developmental Biology, Signal Transduction
Positive Control
Uterus or normal breast
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Non-hazardous. No MSDS required.
SDS-PAGE
(SDS-PAGE Analysis of Purified SM-MHC Monoclonal Antibody (SMMS-1). Confirmation of Integrity and Purity of Antibody)
SDS-PAGE
(SDS-PAGE Analysis of Purified SM-MHC Monoclonal Antibody (SMMS-1). Confirmation of Integrity and Purity of Antibody)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with SM-MHC Monoclonal Antibody (Clone #: SMMS-1).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with SM-MHC Monoclonal Antibody (Clone #: SMMS-1).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with SM-MHC Monoclonal Antibody (Clone#: SMMS-1).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with SM-MHC Monoclonal Antibody (Clone#: SMMS-1).)
References
D.Lazard,.X. Sastre, M.G.Frid, M.A.Glukhova, J.-P. Thiery and V.E.Koteliansky. Expression of smooth muscle-specific proteins in myoepithelium and stromal myofibroblasts of normal and malignant human breast tissue. Proc. Natl. Acad. Sci. USA, 1993, v.90: 999-1003. N.P. Wang, B.C. Wan, M. Skelly, M.G. Frid, M.A. Glukhova, V.E. Koteliansky, A.M. Gown. Antibodies to novel myoepithelium-associated proteins distinquish benign lesions and in-situ- carcinoma from invasive carcinoma of the breast. Applied Immunohistochemistry 1997;5(3):141-151
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
205kDa (MHC-1); 200kDa (MHC-2)
NCBI Official Full Name
myosin-11 isoform SM2B
NCBI Official Synonym Full Names
myosin heavy chain 11
NCBI Official Symbol
MYH11
NCBI Official Synonym Symbols
AAT4; FAA4; SMHC; SMMHC
NCBI Protein Information
myosin-11
UniProt Protein Name
Myosin-11
UniProt Gene Name
MYH11
UniProt Synonym Gene Names
KIAA0866
UniProt Entry Name
MYH11_HUMAN
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Product Notes
The MYH11 myh11 (Catalog #AAA13842) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker) Mouse Monoclonal Antibody reacts with Cat, Chicken, Cow, Dog, Guinea Pig, Human, Pig, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Smooth Muscle Myosin Heavy Chain (SM-MHC) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin. Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the MYH11 myh11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smooth Muscle Myosin Heavy Chain (SM-MHC), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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