IF (Immunofluorescence)
(ICC/IF analysis of SNAP25 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SNAP25 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human, Mouse SNAP25 Monoclonal Antibody | anti-SNAP25 antibody
SNAP25 antibody
Gene Names
SNAP25; SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
SNAP25, Antibody; SNAP25 antibody; Synaptosomal-associated protein 25 isoform SNAP25B; SNAP25; SNAP; Synaptosomal-associated protein; His- tag; SNAP-25; Synaptosomal-associated 25 kDa protein; Super protein; SuP; Synaptosomal-associated protein 25; Synaptosomal-associated protein 25 isoform SNAP25B bA416N4.2; Bdr; dJ1068F16.2; FLJ23079; HGNC:11132; MGC105414; MGC139754; Resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC9; SNAP 25; SNAP-25B; SNP 25; sp; Synaptosomal associated 25 kDa protein; Synaptosomal associated protein; Synaptosomal Associated Protein 25; Synaptosomal associated protein 25kDa.; anti-SNAP25 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
4E11
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
206
Applicable Applications for anti-SNAP25 antibody
WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human SNAP25 (1-206aa) purified from E. coli
Clone Note
Anti-human SNAP25 mAb, clone 4E11, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SNAP25 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of SNAP25 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SNAP25 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of SNAP25 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SNAP25 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The extracts of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human SNAP25 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SNAP25 Transfected 293T cell lysate)
WB (Western Blot)
(The extracts of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human SNAP25 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SNAP25 Transfected 293T cell lysate)
Related Product Information for anti-SNAP25 antibody
The synaptosomal-associated protein (SNAP25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP25 is directly involved in the release of neurotransmitters.
Product Categories/Family for anti-SNAP25 antibody
References
Schulz JR et al., (1998) J Biol Chem 273 (38):24355-9; Tafoya LC et al., (2006) J Neurosci 26 (30):7826-38
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,336 Da
NCBI Official Full Name
synaptosomal-associated protein 25 isoform SNAP25B
NCBI Official Synonym Full Names
synaptosome associated protein 25
NCBI Official Symbol
SNAP25
NCBI Official Synonym Symbols
SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
NCBI Protein Information
synaptosomal-associated protein 25
UniProt Protein Name
Synaptosomal-associated protein 25
UniProt Gene Name
SNAP25
UniProt Synonym Gene Names
SNAP; SNAP-25; SUP
Similar Products
Product Notes
The SNAP25 snap25 (Catalog #AAA48005) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SNAP25 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SNAP25 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SNAP25 snap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNAP25, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.