WB (Western Blot)
(Western blot analysis of JEG-3 cell lysate using SPARC/Osteonectin Mouse Monoclonal Antibody (OSTN/3758).)
Mouse anti-Human SPARC/Osteonectin Monoclonal Antibody | anti-SPARC antibody
SPARC/Osteonectin Mouse Monoclonal Antibody [Clone OSTN/3758]
Gene Names
SPARC; ON
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Synonyms
SPARC/Osteonectin, Antibody; SPARC/Osteonectin Mouse Monoclonal Antibody [Clone OSTN/3758]; Basement membrane protein 40; BM40; Cysteine rich protein; OI17; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and cysteine rich; Secreted protein acidic and rich in cysteine (SPARC); anti-SPARC antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse/IgG1, kappa
Clone Number
OSTN/3758
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-SPARC antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant fragment (around aa1-200) of human SPARC protein (exact sequence is proprietary)
Cellular Localization
Secreted, Basement membrane
Positive Control
Human placenta, lymph node or spleen. JEG3 or A549 cell lysates.
Hu-chromosome Location
5q33.1
Unigene
111779; 708558
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
WB (Western Blot)
(Western blot analysis of JEG-3 cell lysate using SPARC/Osteonectin Mouse Monoclonal Antibody (OSTN/3758).)
WB (Western Blot)
(Western blot analysis of JEG-3 cell lysate using SPARC/Osteonectin Mouse Monoclonal Antibody (OSTN/3758).)
Related Product Information for anti-SPARC antibody
SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,632 Da
NCBI Official Full Name
osteonectin
NCBI Official Synonym Full Names
secreted protein, acidic, cysteine-rich (osteonectin)
NCBI Official Symbol
SPARC
NCBI Official Synonym Symbols
ON
NCBI Protein Information
SPARC; BM-40; osteonectin; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine
UniProt Protein Name
SPARC
UniProt Gene Name
SPARC
UniProt Synonym Gene Names
ON; BM-40; ON
UniProt Entry Name
SPRC_HUMAN
Similar Products
Product Notes
The SPARC sparc (Catalog #AAA215937) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SPARC/Osteonectin Mouse Monoclonal Antibody [Clone OSTN/3758] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPARC/Osteonectin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SPARC sparc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPARC/Osteonectin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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