Mouse anti-Human SPI-C/Transcription factor Spi-C Monoclonal Antibody | anti-SPIC antibody

SPI-C/Transcription factor Spi-C Mouse Monoclonal Antibody [Clone PCRP-SPIC-2C5]

Cat. Num. AAA215557

• Gene Names: SPIC; SPI-C
• Reactivity: Human
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: SPI-C/Transcription factor Spi-C, Antibody; SPI-C/Transcription factor Spi-C Mouse Monoclonal Antibody [Clone PCRP-SPIC-2C5]; AU019198; C76795; MGC40611; MGC80555; Prf; Pu. 1 related factor; Spi C; Spi C transcription factor; Spi-C transcription factor (Spi-1/PU. 1 related); anti-SPIC antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG2b
• Clone Number: PCRP-SPIC-2C5
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-SPIC antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant full-length human SPIC protein
• Cellular Localization: Nucleus
• Positive Control: Jurkat or Raji cells.
• Hu-chromosome Location: 12q23.2
• Unigene: 577097
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. SPIC Mouse Monoclonal Antibody (PCRP-SPIC-2C5) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).)

Related Product Information for anti-SPIC antibody

The Ets transcription factor family is comprised of DNA-binding proteins that influence lymphoid development and activity and bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. Spi-B and Spi-C (also known as SPIC) are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to non-canonical AGAA sites. Spi-C is a 248 amino acid protein that localizes to the nucleus and, like other Ets family members, binds DNA as a monomer and plays a role in transcriptional regulation. Addi- tionally, Spi-C is thought to control the development of red pulp macrophages, thereby contributing to iron homeostasis and red blood cell recycling. Human Spi-C shares 65% amino acid identity with its mouse counterpart, suggesting a conserved role between species.

NCBI and Uniprot Product Information

• NCBI GI #: 22748707
• NCBI GeneID: 121599
• NCBI Accession #: NP_689536.1
• NCBI GenBank Nucleotide #: NM_152323.1
• UniProt Accession #: Q8N5J4
• Molecular Weight: 29,180 Da
• NCBI Official Full Name: transcription factor Spi-C
• NCBI Official Synonym Full Names: Spi-C transcription factor (Spi-1/PU.1 related)
• NCBI Official Symbol: SPIC
• NCBI Official Synonym Symbols: SPI-C
• NCBI Protein Information: transcription factor Spi-C
• UniProt Protein Name: Transcription factor Spi-C
• UniProt Gene Name: SPIC
• UniProt Entry Name: SPIC_HUMAN