IHC (Immunohiostchemistry)
(Immunohistochemistry of SPT5 in paraffin-embedded Human breast cancer tissue using SPT5 Rabbit mAb at dilution 1/50)
SPT5 Monoclonal Antibody | anti-SPT5 antibody
SPT5 Rabbit mAb
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
SPT5, Antibody; SPT5 Rabbit mAb; SPT5; SPT5H; Tat-CT1; anti-SPT5 antibody
Host
Recombinant Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
S01-5C2
Purity/Purification
Affinity Purified
Form/Format
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration
0.3mg/ml (varies by lot)
Applicable Applications for anti-SPT5 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide of human SPT5
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.
IHC (Immunohiostchemistry)
(Immunohistochemistry of SPT5 in paraffin-embedded Human breast cancer tissue using SPT5 Rabbit mAb at dilution 1/50)
IHC (Immunohiostchemistry)
(Immunohistochemistry of SPT5 in paraffin-embedded Human breast cancer tissue using SPT5 Rabbit mAb at dilution 1/50)
WB (Western Blot)
(Western blot detection of SPT5 in Hela,A549,HL-60 cell lysates using SPT5 Rabbit mAb(1:1000 diluted).Predicted band size:121kDa.Observed band size:150kDa.)
WB (Western Blot)
(Western blot detection of SPT5 in Hela,A549,HL-60 cell lysates using SPT5 Rabbit mAb(1:1000 diluted).Predicted band size:121kDa.Observed band size:150kDa.)
Related Product Information for anti-SPT5 antibody
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
Product Categories/Family for anti-SPT5 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The SPT5 supt5h (Catalog #AAA314574) is an Antibody produced from Recombinant Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPT5 Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SPT5 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SPT5 supt5h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPT5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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