Mouse anti-Human, Mouse STAG2 Monoclonal Antibody | anti-STAG2 antibody

Mouse Monoclonal [clone 3C6] (IgG1,k) to Human STAG2

Cat. Num. AAA12354

• Gene Names: STAG2; SA2; SA-2; SCC3B; bA517O1.1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
• Purity: Purified from ascites by Protein A
• Synonyms: STAG2, Antibody; Mouse Monoclonal [clone 3C6] (IgG1,k) to Human STAG2; Anti-STAG2 Antibody (clone 3C6) IHC-plus; STAG2; Cohesin subunit SA-2; SCC3B; SCC3 homolog 2; Stromal antigen 2; SA2; BA517O1.1; SA-2; Human STAG2; anti-STAG2 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3C6
• Specificity: Human Nuclear Protein SA-2
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1231

• Applicable Applications for anti-STAG2 antibody: Western Blot (WB), ELISA (EIA),Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF)
• Application Notes: Western Blot (WB); ELISA (optimal dilution to be determined by the researcher); IHC-P (5 ug/ml); Immunofluorescence (10ug/ml); ; Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Cell lysate, Recombinant protein.
• Immunogen Species: STAG2 antibody was raised against Human
• Antigen type: Recombinant Protein
• Target: STAG2
• Immunogen: STAG2 antibody was raised against sTAG2 (NP_006594, 1130 a.a. ~ 1232 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

WB (Western Blot)

(Western blot of STAG2 expression in HeLa nuclear extract with STAG2 monoclonal antibody, clone 3C6.)

WB (Western Blot)

(Western blot of STAG2 expression in NIH/3T3 cells with STAG2 monoclonal antibody, clone 3C6.)

IF (Immunofluorescence)

(Immunofluorescence of monoclonal antibody to STAG2 on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry)

(Anti-STAG2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 31563531
• NCBI GeneID: 10735
• NCBI Accession #: NP_006594.3
• NCBI GenBank Nucleotide #: NM_006603.4
• UniProt Accession #: Q8N3U4; O00540; Q5JTI6; Q68DE9; Q9H1N8; B1AMT5; D3DTF5
• NCBI Official Full Name: cohesin subunit SA-2 isoform b
• NCBI Official Synonym Full Names: stromal antigen 2
• NCBI Official Symbol: STAG2
• NCBI Official Synonym Symbols: SA2; SA-2; SCC3B; bA517O1.1
• NCBI Protein Information: cohesin subunit SA-2; SCC3 homolog 2
• UniProt Protein Name: Cohesin subunit SA-2
• UniProt Gene Name: STAG2
• UniProt Synonym Gene Names: SA2
• UniProt Entry Name: STAG2_HUMAN