Mouse STAT5A Monoclonal Antibody | anti-STAT5A antibody

Anti-STAT5A Antibody (clone 9F7) IHC-plus

Cat. Num. AAA12377

• Gene Names: STAT5A; MGF; STAT5
• Reactivity: Dog, Rat, Human, Monkey
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G purified
• Synonyms: STAT5A, Antibody; Anti-STAT5A Antibody (clone 9F7) IHC-plus; Mouse Monoclonal [clone 9F7] (IgG1) to Human STAT5A; Human STAT5A; MGF; STAT5; anti-STAT5A antibody

• Host: Mouse
• Reactivity: Dog, Rat, Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9F7
• Specificity: Human STAT5A / STAT5
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 0.73 mg/ml (varies by lot)
• Sequence Length: 794

• Applicable Applications for anti-STAT5A antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:100); IF (1:100); IHC-P (10 ug/ml); WB (1:500 - 1:2000)
• Immunogen: STAT5A antibody was raised against full length human recombinant protein of human STAT5A (NP_003143) produced in HEK293T cell.
• Immunogen Description: Full length human recombinant protein of human STAT5A (NP_003143) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Target Species: Human
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

FCM (Flow Cytometry)

(Flow cytometry of Jurkat cells, using anti-STAT5A antibody (Red), compared to a nonspecific negative control antibody (Blue).)

FCM (Flow Cytometry)

(Flow cytometry of HeLa cells, using anti-STAT5A antibody (Red), compared to a nonspecific negative control antibody (Blue).)

WB (Western Blot)

(Western blot of extracts (35 ug) from 9 different cell lines by using anti-STAT5A monoclonal antibody.)

WB (Western Blot)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT5A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT5A.)

IF (Immunofluorescence)

(Anti-STAT5A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT5A.)

IHC (Immunohistochemistry)

(Anti-STAT5A antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-STAT5A antibody

STAT5A Antibody, MGF Antibody, STAT5 Antibody Description: STAT5A is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones.

NCBI and Uniprot Product Information

• NCBI GI #: 21618342
• NCBI GeneID: 6776
• NCBI Accession #: NP_003143.2
• NCBI GenBank Nucleotide #: NM_003152.3
• UniProt Accession #: P42229; Q1KLZ6
• Molecular Weight: 86,949 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5A isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5A
• NCBI Official Symbol: STAT5A
• NCBI Official Synonym Symbols: MGF; STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5A
• UniProt Protein Name: Signal transducer and activator of transcription 5A
• UniProt Gene Name: STAT5A
• UniProt Synonym Gene Names: STAT5
• UniProt Entry Name: STA5A_HUMAN