STAT5A recombinant antibody

Rabbit anti-STAT5a Recombinant Monoclonal Antibody [BLR124H]

Cat. Num. AAA23841

• Gene Names: STAT5A; MGF; STAT5
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: STAT5a; Monoclonal Recombinant Antibody; Rabbit anti-STAT5a Recombinant Monoclonal Antibody [BLR124H]; signal transducer and activator of transcription 5A; STAT5; epididymis secretory sperm binding protein; MGF; Signal transducer and activator of transcription 5A; STAT5A recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR124H
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 100ug/ml (varies by lot)

• Applicable Applications for STAT5A recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 20 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 ul per 1 x 10^6 cells.
• Immunogen: Between 744 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse STAT5a by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, EL4, RenCa, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)

WB (Western Blot)

(Detection of human STAT5a by western blot. Samples: Whole cell lysate from HeLa, HEK293T, C5/MJ, Hep-G2, CCRF-CEM, MJ, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)

IP (Immunoprecipitation)

(Detection of human STAT5a by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 lot 1) used for IP at 20 ul/mg lysate. STAT5a was also immunoprecipitated by rabbit anti-STAT5a antibody BL-1730A-2D8. For blotting immunoprecipitated STAT5a, AAA23841 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry)

(Detection of mouse STAT5a in FFPE spleen by immunohistochemistry. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human STAT5a in FFPE testicular seminoma by immunohistochemistry. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human STAT5a in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human STAT5a in FFPE CCRF-CEM cells by immunocytochemsitry. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human STAT5a (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Related Product Information for STAT5A recombinant antibody

Signal transducer and activator of transcription 5a (STAT5a) is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones [taken from NCBI Entrez Gene (Gene ID: 6776)].

Product Categories/Family for STAT5A recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 21618342
• NCBI GeneID: 6776
• NCBI Accession #: NP_003143.2
• NCBI GenBank Nucleotide #: NM_003152.3
• UniProt Accession #: P42229
• Molecular Weight: 90,647 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5A
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5A
• NCBI Official Symbol: STAT5A
• NCBI Official Synonym Symbols: MGF; STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5A
• UniProt Protein Name: Signal transducer and activator of transcription 5A
• UniProt Gene Name: STAT5A
• UniProt Synonym Gene Names: STAT5
• UniProt Entry Name: STA5A_HUMAN