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IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Human kidney. 1. Antibody was diluted at 1:200(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
Mouse anti-Human, Rat Survivin Monoclonal Antibody | anti-Survivin antibody
Survivin Monoclonal Antibody (Mix)
Gene Names
BIRC5; API4; EPR-1
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Synonyms
Survivin; Monoclonal Antibody; Survivin Monoclonal Antibody (Mix); Baculoviral IAP repeat-containing protein 5; BIRC5; API4; IAP4; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; anti-Survivin antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Clone Number
Mix
Specificity
The antibody detects endogenous Survivin protein.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Form/Format
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-Survivin antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section)
Application Notes
WB: 1:1000-2000
IHC: 1:200-500
IF: 1:50-200
IHC: 1:200-500
IF: 1:50-200
Immunogen
Recombinant Protein of Survivin
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Human kidney. 1. Antibody was diluted at 1:200(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Human kidney. 1. Antibody was diluted at 1:200(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human kidney. 1. Antibody was diluted at 1:200(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human kidney. 1. Antibody was diluted at 1:200(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon caricnoma using Survivin Monoclonal Antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon caricnoma using Survivin Monoclonal Antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1. Using Survivin Mouse mAb (AAA30823) diluted at 1:200 (4 degree overnight). 2. High-pressure and temperature Citric acid. pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1. Using Survivin Mouse mAb (AAA30823) diluted at 1:200 (4 degree overnight). 2. High-pressure and temperature Citric acid. pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital)
WB (Western Blot)
(Western blot detection of Survivin in human breast cancer cell line MCF-7(A) and Cal51 (B) using Survivin mouse mAb (AAA30823. 1:1000 diluted).Predicted band size: 16kDa.Observed band size:16kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital)
WB (Western Blot)
(Western blot detection of Survivin in human breast cancer cell line MCF-7(A) and Cal51 (B) using Survivin mouse mAb (AAA30823. 1:1000 diluted).Predicted band size: 16kDa.Observed band size:16kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital)
WB (Western Blot)
(Western blot analysis of 1) Hela. 2) 293. 3) PC12 using Survivin Monoclonal Antibody.)
WB (Western Blot)
(Western blot analysis of 1) Hela. 2) 293. 3) PC12 using Survivin Monoclonal Antibody.)
Related Product Information for anti-Survivin antibody
This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, butThis gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
Product Categories/Family for anti-Survivin antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,389 Da
NCBI Official Full Name
baculoviral IAP repeat-containing protein 5 isoform 2
NCBI Official Synonym Full Names
baculoviral IAP repeat containing 5
NCBI Official Symbol
BIRC5
NCBI Official Synonym Symbols
API4; EPR-1
NCBI Protein Information
baculoviral IAP repeat-containing protein 5; apoptosis inhibitor 4; survivin variant 3 alpha; apoptosis inhibitor survivin
UniProt Protein Name
Baculoviral IAP repeat-containing protein 5
UniProt Gene Name
BIRC5
UniProt Synonym Gene Names
API4; IAP4
UniProt Entry Name
BIRC5_HUMAN
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Product Notes
The Survivin birc5 (Catalog #AAA30823) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Survivin Monoclonal Antibody (Mix) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Survivin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section). WB: 1:1000-2000 IHC: 1:200-500 IF: 1:50-200. Researchers should empirically determine the suitability of the Survivin birc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Survivin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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