DB (Dot Blot)
(Dot Blot analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 3D4 (SMC-608). Tissue: Recombinant Protein. Species: Human. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (SMC-608) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking.)
Mouse Tau Monoclonal Antibody | anti-Tau antibody
Tau Antibody, Clone 3D4: HRP
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Immunocytochemistry, Dot Blot, Western Blot
Purity
Protein G Purified
Synonyms
Tau, Antibody; Tau Antibody, Clone 3D4: HRP; Mouse Anti-Human Tau Monoclonal IgG1; Tau Antibody; Neurofibrillary tangle protein Antibody; MAPTL Antibody; Microtubule-associated protein tau Antibody; MTBT1 Antibody; Paired helical filament-tau Antibody; TAU Antibody; PHF-tau Antibody; MAPT Antibody; AI413597 antibody; AW045860 antibody; DDPAC antibody; FLJ31424 antibody; FTDP 17 antibody; G protein beta1/gamma2 subunit interacting factor 1 antibody; MAPT antibody; MAPTL antibody; MGC134287 antibody; MGC138549 antibody; MGC156663 antibody; Microtubule associated protein tau antibody; Microtubule associated protein tau isoform 4 antibody; Microtubule-associated protein tau antibody; MSTD antibody; Mtapt antibody; MTBT1 antibody; MTBT2 antibody; Neurofibrillary tangle protein antibody; Paired helical filament tau antibody; Paired helical filament-tau antibody; PHF tau antibody; PHF-tau antibody; PPND antibody; PPP1R103 antibody; Protein phosphatase 1; regulatory subunit 103 antibody; pTau antibody; RNPTAU antibody; TAU antibody; TAU_HUMAN antibody; Tauopathy and respiratory failure; included antibody; anti-Tau antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3D4
Specificity
Detects Multiple Bands. Antibody detects monomer and fibril under native conditions (dot blot).
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration
1mg/mL (varies by lot)
Sequence Length
776
Applicable Applications for anti-Tau antibody
ELISA, IF (Immunofluorescence), ICC (Immunocytochemistry), DB (Dot Blot), WB (Western Blot)
Immunogen
Human Recombinant Tau441 (2N4R), P301S mutant Protein Pre-formed Fibrils
Immunogen Species
Human
Tissue Specificity
Expressed in neurons.
Conjugate
HRP
Cellular Localization
Cytoplasm, Axolemma, Axolemma Plasma Membrane, Axon, Cell Body, Cell membrane, Cytoplasmic Ribonucleoprotein Granule, Cytoplasmic Side, Cytoskeleton, Cytosol, Dendrite, Growth cone, Microtubule, Microtubule Associated Complex, Neurofibrillary Tangle, Neuronal Cell Body, Nuclear Periphery, Nuclear Speck, Nucleus, Peripheral membrane protein, Plasma Membrane, Tubulin Complex
Certificate of Analysis
A 1:1000 dilution was sufficient for detection of Tau 2N4R P301S Fibril in 20 ug of Mouse Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Preparation and Storage
Store at -20 degree C
Shipping Temperature: Blue Ice or 4 degree C
Shipping Temperature: Blue Ice or 4 degree C
DB (Dot Blot)
(Dot Blot analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 3D4 (SMC-608). Tissue: Recombinant Protein. Species: Human. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (SMC-608) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking.)
DB (Dot Blot)
(Dot Blot analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 3D4 (SMC-608). Tissue: Recombinant Protein. Species: Human. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (SMC-608) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking.)
WB (Western Blot)
(Western Blot analysis of Mouse Brain showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 3D4 (SMC-608). Lane 1: MW Marker. Lane 2: Mouse Brain (20ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (SMC-608) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)
WB (Western Blot)
(Western Blot analysis of Mouse Brain showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 3D4 (SMC-608). Lane 1: MW Marker. Lane 2: Mouse Brain (20ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (SMC-608) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)
Related Product Information for anti-Tau antibody
Background: Alzheimers Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). 2N4R, or Tau-441 is the full length tau protein. P301S is a mutation encoded by exon 10 (4) that impairs the ability of tau to assemble microtubules (5).
Product Categories/Family for anti-Tau antibody
References
1. www.alz.org/alzheimers-dementia/facts-figures
2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146-148 (1907)
3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497.
4. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18.
5. Bugiani, O. et al. (1999). J Neuropathol Exp Neurol. 58(6): 667-77.
2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146-148 (1907)
3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497.
4. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18.
5. Bugiani, O. et al. (1999). J Neuropathol Exp Neurol. 58(6): 667-77.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
microtubule-associated protein tau isoform 6
NCBI Official Synonym Full Names
microtubule associated protein tau
NCBI Official Symbol
MAPT
NCBI Official Synonym Symbols
TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
NCBI Protein Information
microtubule-associated protein tau
UniProt Protein Name
Microtubule-associated protein tau
UniProt Gene Name
MAPT
UniProt Synonym Gene Names
MAPTL; MTBT1; TAU; PHF-tau
UniProt Entry Name
TAU_HUMAN
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Product Notes
The Tau mapt (Catalog #AAA253995) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Tau Antibody, Clone 3D4: HRP reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tau can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), ICC (Immunocytochemistry), DB (Dot Blot), WB (Western Blot). Researchers should empirically determine the suitability of the Tau mapt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tau, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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