IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with TGF alpha Mouse Monoclonal Antibody (MF9))
Mouse TGF-alpha Monoclonal Antibody | anti-TGFA antibody
TGF-alpha
Gene Names
TGFA; TFGA
Reactivity
Human, Cow, Rat, Mouse.
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Synonyms
TGF-alpha, Antibody; TGF-alpha; EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Waved 1; anti-TGFA antibody
Host
Mouse
Reactivity
Human, Cow, Rat, Mouse.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
MF9
Specificity
This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGFalpha (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
159
Applicable Applications for anti-TGFA antibody
IHC (Immunohistochemistry), WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA
Cellular Localization
Cell membrane, Extracellular space, Secreted
Positive Control
Jurkat or Ramos cells. Testicular Carcinoma.
Human Chromosomal Location
2p13.3
Immunogen
A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFalpha.
Preparation and Storage
Antibody without azide - store at -20 to -80°C. Non-hazardous. No MSDS required.
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with TGF alpha Mouse Monoclonal Antibody (MF9))
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with TGF alpha Mouse Monoclonal Antibody (MF9))
SDS-PAGE
(SDS-PAGE Analysis of Purified TGF alpha Mouse Monoclonal Antibody (MF9). Confirmation of Integrity and Purity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis of Purified TGF alpha Mouse Monoclonal Antibody (MF9). Confirmation of Integrity and Purity of Antibody.)
References
Kobrin MS et. al. J Bio Chem, 1986, 261:14414-9.
2. Kudlow JE et. al. Endocrinology, 1987, 121(4):1577-9.
3. Kobrin MS et. al. Endocrinology, 1987, 121(4):1412-6.
4. Kudlow JE et. al. J Bio Chem, 1989, 264(7):3880-3
2. Kudlow JE et. al. Endocrinology, 1987, 121(4):1577-9.
3. Kobrin MS et. al. Endocrinology, 1987, 121(4):1412-6.
4. Kudlow JE et. al. J Bio Chem, 1989, 264(7):3880-3
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~6kDa
NCBI Official Full Name
protransforming growth factor alpha isoform 2 preproprotein
NCBI Official Synonym Full Names
transforming growth factor, alpha
NCBI Official Symbol
TGFA
NCBI Official Synonym Symbols
TFGA
NCBI Protein Information
protransforming growth factor alpha; TGF-alpha
UniProt Protein Name
Protransforming growth factor alpha
UniProt Gene Name
Tgfa
UniProt Synonym Gene Names
TGF-alpha; ETGF
UniProt Entry Name
TGFA_MOUSE
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Product Notes
The TGFA tgfa (Catalog #AAA62554) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TGF-alpha reacts with Human, Cow, Rat, Mouse. and may cross-react with other species as described in the data sheet. AAA Biotech's TGF-alpha can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the TGFA tgfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGF-alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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