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product-image-AAA11051_FCM6.gif FCM (Flow Cytometry) (Flow cytometry analysis of TIGIT overexpressing HEK293 cells using TIGIT single domain antibody and control 1H4 single domain antibody at 0.05ug/ml. Blue: Untransfected HEK293 cells. Yellow: TIGIT overexpressing HEK293 cells.)

TIGIT recombinant antibody

TIGIT Single Domain Antibody [2F7]

Gene Names
TIGIT; VSIG9; VSTM3; WUCAM
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
TIGIT Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.  It also contains a myc-tag for detection.
Synonyms
TIGIT, Recombinant Antibody; TIGIT Single Domain Antibody [2F7]; TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; WUCAM; T-cell immunoreceptor with Ig and ITIM domains; TIGIT recombinant antibody
Ordering
For Research Use Only!
Host
Llama
Reactivity
Human
Clonality
Monoclonal
Clone Number
2F7
Purity/Purification
TIGIT Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.  It also contains a myc-tag for detection.
Form/Format
Liquid; TIGIT Antibody is supplied in PBS.
Concentration
1mg/mL (varies by lot)
Applicable Applications for TIGIT recombinant antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
TIGIT antibody can be used for ELISA starting at 0.1ug/mLimmunohistochemistry starting at 10ug/mL. For immunofluorescence start at 20ug/mL. For flow cytometry at 1ug/ml. For immunocytochemistry at 10ug/mL.
Immunogen
TIGIT antibody was raised against a recombinant protein corresponding to amino acids 22-141 of human TIGIT.
Research Area
Immunology
NCBI Organism
Homo sapiens
Preparation and Storage
TIGIT antibody should be stored in working aliquots at -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

FCM (Flow Cytometry)

(Flow cytometry analysis of TIGIT overexpressing HEK293 cells using TIGIT single domain antibody and control 1H4 single domain antibody at 0.05ug/ml. Blue: Untransfected HEK293 cells. Yellow: TIGIT overexpressing HEK293 cells.)

product-image-AAA11051_FCM6.gif FCM (Flow Cytometry) (Flow cytometry analysis of TIGIT overexpressing HEK293 cells using TIGIT single domain antibody and control 1H4 single domain antibody at 0.05ug/ml. Blue: Untransfected HEK293 cells. Yellow: TIGIT overexpressing HEK293 cells.)

IHC (Immunohistochemistry)

(Immunohistochemistry of TIGIT in human spleen tissue with TIGIT single domain antibody at 1ug/mL.)

product-image-AAA11051_IHC5.gif IHC (Immunohistochemistry) (Immunohistochemistry of TIGIT in human spleen tissue with TIGIT single domain antibody at 1ug/mL.)

ELISA

(Titration ELISA analysis of TIGIT sdAbs to detect recombinant TIGIT (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.)

product-image-AAA11051_ELISA4.gif ELISA (Titration ELISA analysis of TIGIT sdAbs to detect recombinant TIGIT (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.)

IF (Immunofluorescence)

(Immunofluorescence of TIGIT in human spleen tissue with TIGIT single domain antibody at 20ug/mL.)

product-image-AAA11051_IF3.gif IF (Immunofluorescence) (Immunofluorescence of TIGIT in human spleen tissue with TIGIT single domain antibody at 20ug/mL.)

IF (Immunofluorescence)

(Immunofluorescence of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 20ug/mL.)

product-image-AAA11051_IF2.gif IF (Immunofluorescence) (Immunofluorescence of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 20ug/mL.)

ICC (Immunocytochemistry)

(Immunocytochemistry of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 10ug/mL.)

product-image-AAA11051_ICC.gif ICC (Immunocytochemistry) (Immunocytochemistry of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 10ug/mL.)
Related Product Information for TIGIT recombinant antibody
TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).
Product Categories/Family for TIGIT recombinant antibody
References
Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63.
Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8(+) T cell effector function. Cancer Cell 2014; 26:923-37.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 26 kDa
NCBI Official Full Name
T-cell immunoreceptor with Ig and ITIM domains
NCBI Official Synonym Full Names
T cell immunoreceptor with Ig and ITIM domains
NCBI Official Symbol
TIGIT
NCBI Official Synonym Symbols
VSIG9; VSTM3; WUCAM
NCBI Protein Information
T-cell immunoreceptor with Ig and ITIM domains
UniProt Protein Name
T-cell immunoreceptor with Ig and ITIM domains
UniProt Gene Name
TIGIT
UniProt Synonym Gene Names
VSIG9; VSTM3

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Product Notes

The TIGIT tigit (Catalog #AAA11051) is a Recombinant Antibody produced from Llama and is intended for research purposes only. The product is available for immediate purchase. The TIGIT Single Domain Antibody [2F7] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIGIT can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). TIGIT antibody can be used for ELISA starting at 0.1ug/mLimmunohistochemistry starting at 10ug/mL. For immunofluorescence start at 20ug/mL. For flow cytometry at 1ug/ml. For immunocytochemistry at 10ug/mL. Researchers should empirically determine the suitability of the TIGIT tigit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIGIT, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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