TIGIT recombinant antibody

TIGIT Single Domain Antibody [2F7]

Cat. Num. AAA11051

• Gene Names: TIGIT; VSIG9; VSTM3; WUCAM
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: TIGIT Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
• Synonyms: TIGIT, Recombinant Antibody; TIGIT Single Domain Antibody [2F7]; TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; WUCAM; T-cell immunoreceptor with Ig and ITIM domains; TIGIT recombinant antibody

• Host: Llama
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: 2F7
• Purity/Purification: TIGIT Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
• Form/Format: Liquid; TIGIT Antibody is supplied in PBS.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for TIGIT recombinant antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: TIGIT antibody can be used for ELISA starting at 0.1ug/mLimmunohistochemistry starting at 10ug/mL. For immunofluorescence start at 20ug/mL. For flow cytometry at 1ug/ml. For immunocytochemistry at 10ug/mL.
• Immunogen: TIGIT antibody was raised against a recombinant protein corresponding to amino acids 22-141 of human TIGIT.
• Research Area: Immunology
• NCBI Organism: Homo sapiens
• Preparation and Storage: TIGIT antibody should be stored in working aliquots at -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

FCM (Flow Cytometry)

(Flow cytometry analysis of TIGIT overexpressing HEK293 cells using TIGIT single domain antibody and control 1H4 single domain antibody at 0.05ug/ml. Blue: Untransfected HEK293 cells. Yellow: TIGIT overexpressing HEK293 cells.)

IHC (Immunohistochemistry)

(Immunohistochemistry of TIGIT in human spleen tissue with TIGIT single domain antibody at 1ug/mL.)

ELISA

(Titration ELISA analysis of TIGIT sdAbs to detect recombinant TIGIT (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.)

IF (Immunofluorescence)

(Immunofluorescence of TIGIT in human spleen tissue with TIGIT single domain antibody at 20ug/mL.)

IF (Immunofluorescence)

(Immunofluorescence of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 20ug/mL.)

ICC (Immunocytochemistry)

(Immunocytochemistry of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 10ug/mL.)

Related Product Information for TIGIT recombinant antibody

TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).

Product Categories/Family for TIGIT recombinant antibody

Primary Antibodies

References

Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63.
Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8(+) T cell effector function. Cancer Cell 2014; 26:923-37.

NCBI and Uniprot Product Information

• NCBI GI #: 256600228
• NCBI GeneID: 201633
• NCBI Accession #: NP_776160
• NCBI GenBank Nucleotide #: NM_173799.3
• UniProt Accession #: Q495A1; Q495A3; Q5JPD8; Q6MZS2; Q8N877
• Molecular Weight: Predicted: 26 kDa
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: TIGIT
• NCBI Official Synonym Symbols: VSIG9; VSTM3; WUCAM
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Protein Name: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Gene Name: TIGIT
• UniProt Synonym Gene Names: VSIG9; VSTM3