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Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using TLE1 Mouse Monoclonal Antibody (TLE1/2085).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse TLE1 Monoclonal Antibody | anti-TLE1 antibody
TLE1 (Synovial Sarcoma Marker)
Gene Names
TLE1; ESG; ESG1; GRG1
Reactivity
Human. Others not known.
Applications
Immunohistochemistry
Synonyms
TLE1, Antibody; TLE1 (Synovial Sarcoma Marker); Enhancer of split groucho 1 (ESG1); Enhancer of split groucho-like protein 1; GRG1; TLE1; Transducin like enhancer of split 1; Transducin-like enhancer protein 1; anti-TLE1 antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
TLE1/2085
Specificity
Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein 1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
780
Applicable Applications for anti-TLE1 antibody
IHC (Immunohistochemistry)
Immunogen
Recombinant fragment (aa 175-338) of human TLE1
Cellular Localization
Nuclear
Hu-chromosome Location
9q21.32
Positive Control
HeLa, HepG2 or Jurkat cells. Endometrium Carcinoma or Sarcomas
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using TLE1 Mouse Monoclonal Antibody (TLE1/2085).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using TLE1 Mouse Monoclonal Antibody (TLE1/2085).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
WB (Western Blot)
(Western Blot Analysis of human HeLa Cell lysate using TLE1 Mouse Monoclonal Antibody (TLE1/2085).)
WB (Western Blot)
(Western Blot Analysis of human HeLa Cell lysate using TLE1 Mouse Monoclonal Antibody (TLE1/2085).)
SDS-PAGE
(SDS-PAGE Analysis Purified TLE1 Mouse Monoclonal Antibody (TLE1/2085).Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified TLE1 Mouse Monoclonal Antibody (TLE1/2085).Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded Human Endometrial Carcinoma stained with TLE1 Mouse Monoclonal Antibody (TLE1/2085).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded Human Endometrial Carcinoma stained with TLE1 Mouse Monoclonal Antibody (TLE1/2085).)
References
Jagdis A, et al. Prospective evaluation of TLE1 as a diagnostic immunohistochemical marker in synovial sarcoma. Am J Surg Pathol. 2009; 33:1743-51.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83kDa
NCBI Official Full Name
transducin-like enhancer protein 1 isoform 1
NCBI Official Synonym Full Names
transducin like enhancer of split 1
NCBI Official Symbol
TLE1
NCBI Official Synonym Symbols
ESG; ESG1; GRG1
NCBI Protein Information
transducin-like enhancer protein 1
UniProt Protein Name
Transducin-like enhancer protein 1
UniProt Gene Name
TLE1
UniProt Synonym Gene Names
ESG1
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Product Notes
The TLE1 tle1 (Catalog #AAA214701) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TLE1 (Synovial Sarcoma Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's TLE1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the TLE1 tle1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLE1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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