Mouse anti-Human TLR2 TLR2 Monoclonal Antibody | anti-TLR2 antibody

TLR2, Mouse, mAb T2.5

Cat. Num. AAA77782

• Gene Names: TLR2; TIL4; CD282
• Reactivity: Human TLR2
• Applications: Immunoprecipitation, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Protein G
• Synonyms: TLR2, Antibody; TLR2, Mouse, mAb T2.5; TLR2, CD282, TIL4; anti-TLR2 antibody

• Host: Mouse
• Reactivity: Human TLR2
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Purity/Purification: Protein G
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentratrion is <24 EU/mg, determined with LAL Assay.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Concentration: 100ug/ml (varies by lot)
• Sequence Length: 784

• Applicable Applications for anti-TLR2 antibody: IP (Immunoprecipitation), IF (Immunofluorescence), IA (Immunoassay), FCM/FACS (Flow Cytometry)
• Immunogen: Mouse TLR2 peptide
• Endotoxin: <0.4EU/mg
• Positive Control: RAW264.7 cells
• Negative Control: CHO cells.
• Preparation and Storage: Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.

Application Data

(FC: detection of TLR2 in RAW cells. Red, black and blue line represent the isotype control, cells only and AAA77782 with a concentration of 10 ug/ml, respectively.)

Application Data

Related Product Information for anti-TLR2 antibody

Product Description: The monoclonal antibody T2.5 recognizes human Toll-like receptor 2 (TLR2). Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. At present, ligands for several of the TLR's, such as TLR2-6,9, have been identified, confirming their role in first line defense against invading microorganism. In mammals, TLRs are identified as type I transmembrane signaling receptors with an extracellular portion containing leucine-rich repeats with pattern recognition capabilities. Pathogen recognition by TLRs provokes rapid activation of innate immunity by inducing proliferation of proinflammatory cytokines and upregulation of costimulatory molecules and eventually toinitiation of adaptive immunity. TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. It is suggested that TLR2 is able to recognize such a wide variety of PAMPs (pathogen-specific molecular patterns) by forming heterodimers with other TLRs like e.g. TLR6. TLR2 is essential for recognizing lipopeptides and lipoproteins from several microorganisms and also peptidoglycans derived from gram-positive bacteria. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2.

References

1. Meng, G et al; Antagonistic antibody prevents toll-like receptor 2-driven lethal shock-like syndromes. J Clin Invest 2004, 113: 1473 2. Roura-Mir, C et al; Mycobacterium tuberculosis regulates CD1 antigen presentation pathways through TLR-2. J Immunol 2005, 175: 1758
3. Leemans, J et al; Renal-associated TLR2 mediates ischemia/reperfusion injury in the kidney. J Clin Invest 2005 115: 2894
4. Spiller, S et al; TLR4-induced IFN-γ production increases TLR2 sensitivity and drives Gram-negative sepsis in mice. JEM 2008, 205: 1747
5. Sutmuller, R et al; Toll-like receptor 2 controls expansion and function of regulatory T-cells. J Clin Invest 2006, 116:485
6. Brull, F et al; TLR2 activation is essential to induce a Th1 shift in human peripheral blood mononuclear cells by plant stanols and plant sterols. J Biol Chem 2010, 285:2951
7. Guan, Y et al; Human TLRs 10 and 1 share common mechanism of innate immune sensing but not signaling. J Immunol 2010, 184:5094

NCBI and Uniprot Product Information

• NCBI GI #: 19718734
• NCBI GeneID: 7097
• NCBI Accession #: NP_003255.2
• NCBI GenBank Nucleotide #: NM_003264.3
• NCBI Official Full Name: toll-like receptor 2
• NCBI Official Synonym Full Names: toll-like receptor 2
• NCBI Official Symbol: TLR2
• NCBI Official Synonym Symbols: TIL4; CD282
• NCBI Protein Information: toll-like receptor 2; toll/interleukin 1 receptor-like 4; toll/interleukin-1 receptor-like protein 4
• UniProt Protein Name: Toll-like receptor 2
• UniProt Gene Name: TLR2
• UniProt Synonym Gene Names: TIL4
• UniProt Entry Name: TLR2_HUMAN