FCM/FACS (Flow Cytometry)
(Fig-1: Intracellular flow analysis of hTLR4 APC conjugated antibody in hPBMC (Monocytes) using 0.5 ug/10^6 cells of hTLR4 antibody (Clone: HTA125). Green represents isotype control; red represents anti-hTLR4 APC conjugated antibody.)
Mouse anti-Human TLR4 Monoclonal Antibody | anti-TLR4 antibody
Monoclonal Antibody to TLR4 (Clone: HTA125)
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein G Chromatography
Synonyms
TLR4, Antibody; Monoclonal Antibody to TLR4 (Clone: HTA125); TLR4; anti-TLR4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
HTA125
Purity/Purification
Protein G Chromatography
Form/Format
0.2 mg/ml in PBS with 0.05% Azide.
Sequence Length
839
Applicable Applications for anti-TLR4 antibody
FCM/FACS (Flow Cytometry)
Immunogen Information
This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.
Antigen
TLR4
Conjugate
APC
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
FCM/FACS (Flow Cytometry)
(Fig-1: Intracellular flow analysis of hTLR4 APC conjugated antibody in hPBMC (Monocytes) using 0.5 ug/10^6 cells of hTLR4 antibody (Clone: HTA125). Green represents isotype control; red represents anti-hTLR4 APC conjugated antibody.)
FCM/FACS (Flow Cytometry)
(Fig-1: Intracellular flow analysis of hTLR4 APC conjugated antibody in hPBMC (Monocytes) using 0.5 ug/10^6 cells of hTLR4 antibody (Clone: HTA125). Green represents isotype control; red represents anti-hTLR4 APC conjugated antibody.)
Related Product Information for anti-TLR4 antibody
TLR4 (Toll-like receptor-4) is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. It primarily mediates cellular signaling induced by Gram-negative bacteria. TLR4 is highly specific for LPS and it is associated with CD14, a co-receptor for LPS. The downstream signaling pathway of TLR4 leads to the activation of NF-kappaB through myeloid differentiation protein (MyD88) and IL-1 receptor-associated kinase in various cell types. Keratinocytes express TLR4 at the mRNA and protein levels whereas TLR4 are also present in the normal human epidermis in vivo and their expression is regulated by microbial components. TLR4 is expressed on CD4+ T cells as well, the functional significance of which is unclear.
Product Categories/Family for anti-TLR4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
73,301 Da
NCBI Official Full Name
Toll-like receptor 4
NCBI Official Synonym Full Names
toll like receptor 4
NCBI Official Symbol
TLR4
NCBI Official Synonym Symbols
TOLL; CD284; TLR-4; ARMD10
NCBI Protein Information
toll-like receptor 4
UniProt Protein Name
Toll-like receptor 4
UniProt Gene Name
TLR4
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Product Notes
The TLR4 tlr4 (Catalog #AAA78288) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to TLR4 (Clone: HTA125) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TLR4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the TLR4 tlr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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