<
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with TNFAIP3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Rabbit anti-Human TNFAIP3 Monoclonal Antibody | anti-TNFAIP3 antibody
TNFAIP3 Antibody
Gene Names
TNFAIP3; A20; OTUD7C; TNFA1P2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
TNFAIP3; Monoclonal Antibody; TNFAIP3 Antibody; A20 antibody; AISBL antibody; MGC104522 antibody; MGC138687 antibody; MGC138688 antibody; OTU domain containing protein 7C antibody; OTU domain-containing protein 7C antibody; OTUD7C antibody; Putative DNA binding protein A20 antibody; Putative DNA-binding protein A20 antibody; TNAP3_HUMAN antibody; TNF alpha-induced protein 3 antibody; TNFA1P2 antibody; TNFAIP 3 antibody; TNFAIP3 (A20) antibody; TNFAIP3 antibody; Tumor necrosis factor alpha induced protein 3 antibody; Tumor necrosis factor alpha-induced protein 3 antibody; Tumor necrosis factor induced protein 3 antibody; Tumor necrosis factor inducible protein A20 antibody; tumor necrosis factor; alpha-induced protein 3 antibody; Zinc finger protein A20 antibody; anti-TNFAIP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
SN07-31
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-TNFAIP3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with TNFAIP3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with TNFAIP3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry)
(ICC staining TNFAIP3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TNFAIP3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TNFAIP3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TNFAIP3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TNFAIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining TNFAIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TNFAIP3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TNFAIP3 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of TNFAIP3 on Jurkat cells lysates using anti-TNFAIP3 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of TNFAIP3 on Jurkat cells lysates using anti-TNFAIP3 antibody at 1/1, 000 dilution.)
Related Product Information for anti-TNFAIP3 antibody
A20 is a Cys2/Cys2 zinc finger protein that is induced by a variety of inflammatory stimuli and regulates gene expression. Specifically, A20 is induced by tumor necrosis factor (TNF) and interleukin 1 (IL-1), and acts as a negative regulator of nuclear factor kappa B (NFkappaB) gene expression. By inhibiting NFkappaB activation, A20 plays a critical role in terminating NFkappaB responses to various stimuli. Although the C-terminal region of A20 contains seven zinc finger domains, only four of these domains are required for in vitro inhibition of TNF-induced NFkappaB activation. A20 also interacts with several other proteins, such as TRAF2, TRAF6 and IkappaB kinase (IKK) gamma protein, and can thereby inhibit cell death. TXBP151, a novel A20-binding protein, may mediate the anti-apoptotic activity of A20. Involved in the negative feedback regulation of signal transduction, A20 and A20-binding proteins may be useful as novel therapeutic tools in the treatment of a variety of diseases.
Product Categories/Family for anti-TNFAIP3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,614 Da
NCBI Official Full Name
tumor necrosis factor alpha-induced protein 3
NCBI Official Synonym Full Names
tumor necrosis factor, alpha-induced protein 3
NCBI Official Symbol
TNFAIP3
NCBI Official Synonym Symbols
A20; OTUD7C; TNFA1P2
NCBI Protein Information
tumor necrosis factor alpha-induced protein 3; zinc finger protein A20; TNF alpha-induced protein 3; OTU domain-containing protein 7C; putative DNA-binding protein A20; tumor necrosis factor inducible protein A20
UniProt Protein Name
Tumor necrosis factor alpha-induced protein 3
UniProt Gene Name
TNFAIP3
UniProt Synonym Gene Names
OTUD7C; TNF alpha-induced protein 3
UniProt Entry Name
TNAP3_HUMAN
Similar Products
Product Notes
The TNFAIP3 tnfaip3 (Catalog #AAA30161) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TNFAIP3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TNFAIP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the TNFAIP3 tnfaip3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNFAIP3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
