FCM/FACS (Flow Cytometry)
(Overlay histogram showing 293F cells transfected with TNFRSF17 stained with AAA278884 (red line) at 1:100. The cells were fixed in 70% ethanol at 4 degree C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Mouse anti-Human TNFRSF17 Monoclonal Antibody | anti-TNFRSF17 antibody
TNFRSF17 Monoclonal Antibody
Gene Names
TNFRSF17; BCM; BCMA; CD269; TNFRSF13A
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
TNFRSF17, Antibody; TNFRSF17 Monoclonal Antibody; B cell maturation antigen antibody; B cell maturation factor antibody; B cell maturation protein antibody; B-cell maturation protein antibody; BCM antibody; BCMA antibody; CD269 antibody; CD269 antigen antibody; TNFRSF17 antibody; TNR17_HUMAN antibody; Tumor necrosis factor receptor superfamily member 17 antibody; anti-TNFRSF17 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
10A9F7
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-TNFRSF17 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
Recombinant Human TNFRSF17 protein (1-54AA)
Conjugation
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay histogram showing 293F cells transfected with TNFRSF17 stained with AAA278884 (red line) at 1:100. The cells were fixed in 70% ethanol at 4 degree C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing 293F cells transfected with TNFRSF17 stained with AAA278884 (red line) at 1:100. The cells were fixed in 70% ethanol at 4 degree C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence)
(Immunofluorescence staining of 293F cells transfected with TNFRSF17 with AAA278884 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of 293F cells transfected with TNFRSF17 with AAA278884 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).)
WB (Western Blot)
(Western BlotPositive WB detected in: 1-2 lanes: 293F whole cell lysate transfected with BCMA; 3-4 lane: Recombinant proteins with BCMAAll lanes: TNFRSF17 antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 40, 15 KDaObserved band size: 40, 15 KDaExposure time?10min)
WB (Western Blot)
(Western BlotPositive WB detected in: 1-2 lanes: 293F whole cell lysate transfected with BCMA; 3-4 lane: Recombinant proteins with BCMAAll lanes: TNFRSF17 antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 40, 15 KDaObserved band size: 40, 15 KDaExposure time?10min)
Product Categories/Family for anti-TNFRSF17 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,843 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 17
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 17
NCBI Official Symbol
TNFRSF17
NCBI Official Synonym Symbols
BCM; BCMA; CD269; TNFRSF13A
NCBI Protein Information
tumor necrosis factor receptor superfamily member 17; B cell maturation antigen; B-cell maturation factor; B-cell maturation protein
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 17
UniProt Gene Name
TNFRSF17
UniProt Synonym Gene Names
BCM; BCMA
UniProt Entry Name
TNR17_HUMAN
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Product Notes
The TNFRSF17 tnfrsf17 (Catalog #AAA278884) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TNFRSF17 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TNFRSF17 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TNFRSF17 tnfrsf17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNFRSF17, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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