IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat heart using TOM20 Rabbit mAb (AAA28304) at dilution of 1:50(40xlens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
Rabbit TOM20 Monoclonal Antibody | anti-TOM20 antibody
TOM20 Rabbit mAb
IHC 1:50 - 1:200
IF/ICC 1:100 - 1:500
IP 1:500 - 1:1000
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat heart using TOM20 Rabbit mAb (AAA28304) at dilution of 1:50(40xlens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat heart using TOM20 Rabbit mAb (AAA28304) at dilution of 1:50(40xlens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using TOM20 Rabbit mAb (AAA28304) at dilution of 1:50(40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using TOM20 Rabbit mAb (AAA28304) at dilution of 1:50(40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta using TOM20 Rabbit mAb (AAA28304) at dilution of 1:50(40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human placenta using TOM20 Rabbit mAb (AAA28304) at dilution of 1:50(40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using TOM20 Rabbit mAb (AAA28304) at dilution of 1:50(40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using TOM20 Rabbit mAb (AAA28304) at dilution of 1:50(40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug TOM20 antibody. Western blot was performed from the immunoprecipitate using TOM20 antibody at a dilition of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug TOM20 antibody. Western blot was performed from the immunoprecipitate using TOM20 antibody at a dilition of 1:1000.)
IF (Immunofluorescence)
(Confocal imaging of HeLa cells using TOM20 Rabbit mAb (AAA28304,dilution 1:100) (Red).The cells were counterstained with α-Tubulin Mouse mAb (,dilution 1:400) (Green).DAPI was used for nuclear staining (blue). Objective: 60x.)
IF (Immunofluorescence)
(Confocal imaging of HeLa cells using TOM20 Rabbit mAb (AAA28304,dilution 1:100) (Red).The cells were counterstained with α-Tubulin Mouse mAb (,dilution 1:400) (Green).DAPI was used for nuclear staining (blue). Objective: 60x.)
IF (Immunofluorescence)
(Confocal imaging of HeLa cells using TOM20 Rabbit mAb (AAA28304,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.)
IF (Immunofluorescence)
(Confocal imaging of HeLa cells using TOM20 Rabbit mAb (AAA28304,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using TOM20 Rabbit mAb (AAA28304) at dilution of 1:400 (40xlens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using TOM20 Rabbit mAb (AAA28304) at dilution of 1:400 (40xlens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using TOM20 Rabbit mAb (AAA28304) at dilution of 1:400 (40xlens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using TOM20 Rabbit mAb (AAA28304) at dilution of 1:400 (40xlens). Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and TOM20 knockdown (KD) 293T, using TOM20 Rabbit pAb antibody (AAA28304) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and TOM20 knockdown (KD) 293T, using TOM20 Rabbit pAb antibody (AAA28304) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TOM20 antibody (AAA28304) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TOM20 antibody (AAA28304) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TOM20 antibody (AAA28304) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TOM20 antibody (AAA28304) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TOM20 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TOM20 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.)
NCBI and Uniprot Product Information
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Product Notes
The TOM20 (Catalog #AAA28304) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TOM20 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB 1:2000 - 1:6000 IHC 1:50 - 1:200 IF/ICC 1:100 - 1:500 IP 1:500 - 1:1000. Researchers should empirically determine the suitability of the TOM20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOM20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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