IF (Immunofluorescence)
(Immunofluorescence of Topoisomerase I(green) in Hela cells using Topoisomerase I Rabbit mAb at dilution 1:200, and DAPI(blue))
Rabbit anti-Human, Mouse Topoisomerase I Monoclonal Antibody | anti-TOP1 antibody
Recombinant Topoisomerase I Monoclonal Antibody
Gene Names
TOP1; TOPI
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
Topoisomerase I, Antibody; Recombinant Topoisomerase I Monoclonal Antibody; TOP1; TOPI; anti-TOP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
R02-2H9
Purity/Purification
Affinity Purified
Form/Format
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Concentration
300ug/mL (varies by lot)
Applicable Applications for anti-TOP1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide of human Topoisomerase I
Preparation and Storage
Storage: -20 degree C/One year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence of Topoisomerase I(green) in Hela cells using Topoisomerase I Rabbit mAb at dilution 1:200, and DAPI(blue))
IF (Immunofluorescence)
(Immunofluorescence of Topoisomerase I(green) in Hela cells using Topoisomerase I Rabbit mAb at dilution 1:200, and DAPI(blue))
IHC (Immunohiostchemistry)
(Immunohistochemistry of Topoisomerase I in paraffin-embedded Human colon cancer tissue using Topoisomerase I Rabbit mAb at dilution 1:50)
IHC (Immunohiostchemistry)
(Immunohistochemistry of Topoisomerase I in paraffin-embedded Human colon cancer tissue using Topoisomerase I Rabbit mAb at dilution 1:50)
WB (Western Blot)
(Western blot detection of Topoisomerase I in K562,Rat Brain,C6,3T3,Hela cell lysates using Topoisomerase I Rabbit mAb(1:1000 diluted).Predicted band size:91kDa.Observed band size:91kDa.)
WB (Western Blot)
(Western blot detection of Topoisomerase I in K562,Rat Brain,C6,3T3,Hela cell lysates using Topoisomerase I Rabbit mAb(1:1000 diluted).Predicted band size:91kDa.Observed band size:91kDa.)
Related Product Information for anti-TOP1 antibody
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Product Categories/Family for anti-TOP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90,726 Da
NCBI Official Full Name
DNA topoisomerase 1
NCBI Official Synonym Full Names
topoisomerase (DNA) I
NCBI Official Symbol
TOP1
NCBI Official Synonym Symbols
TOPI
NCBI Protein Information
DNA topoisomerase 1; type I DNA topoisomerase
UniProt Protein Name
DNA topoisomerase 1
UniProt Gene Name
TOP1
UniProt Entry Name
TOP1_HUMAN
Similar Products
Product Notes
The TOP1 top1 (Catalog #AAA178809) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Topoisomerase I Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Topoisomerase I can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TOP1 top1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Topoisomerase I, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.