IF (Immunofluorescence)
(Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3))
Mouse anti-Human TRA1 Monoclonal Antibody | anti-TRA1 antibody
TRA1 antibody
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human
Applications
Immunofluorescence, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
TRA1, Antibody; TRA1 antibody; 94 kDa glucose-regulated protein; HSP90B1; GP96; TRA1; Tumor rejection antigen 1; GRP94; 94 kDa glucose-regulated protein 94 kDa glucose regulated protein; ECGP; Endoplasmin; Endothelial cell (HBMEC) glycoprotein; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 9; Heat shock protein 90 kDa beta member 1; Stress inducible tumor rejection antigen GP96; Tumor rejection antigen gp96.; anti-TRA1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
2H3
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
803
Applicable Applications for anti-TRA1 antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human TRA1 (676-803aa) purified from E. coli
Clone Note
Anti-human TRA1 mAb, clone 2H3, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TRA1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3))
IF (Immunofluorescence)
(Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3))
WB (Western Blot)
(The cell lysates of hES (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TRA1 (1:500). Protein were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : ES cell lysate)
WB (Western Blot)
(The cell lysates of hES (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TRA1 (1:500). Protein were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : ES cell lysate)
Related Product Information for anti-TRA1 antibody
Tumor rejection antigen 1 (TRA1) is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA1 is a marker protein of these stem cells.
Product Categories/Family for anti-TRA1 antibody
References
Wulf GM, et al., (2002) J Biol. Chem. 277 (50)47976-47979; Hamdane M, et al., (2002) J Mol Neurosci. 19 (3) ; 275-287 Zheng H, et al., (2002) Nature 419 (6909) 853-857; Lu,k.P. et al., (1996) Nature 380 (6574) 544-547.; Campbell,H.D, et al., (1997) Genomics 44 (2), 157-162
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,469 Da
NCBI Official Full Name
endoplasmin
NCBI Official Synonym Full Names
heat shock protein 90 beta family member 1
NCBI Official Symbol
HSP90B1
NCBI Official Synonym Symbols
ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
NCBI Protein Information
endoplasmin
UniProt Protein Name
Endoplasmin
UniProt Gene Name
HSP90B1
UniProt Synonym Gene Names
GRP94; TRA1; GRP-94
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Product Notes
The TRA1 hsp90b1 (Catalog #AAA47994) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TRA1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRA1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TRA1 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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