SDS-PAGE
(SDS-PAGE Analysis of Purified TRAcP Rabbit Recombinant Monoclonal Antibody (ACP5/2336R).)
Rabbit TRAcP (Tartrate-Resistant Acid Phosphatase) Monoclonal Antibody | anti-TRAcP antibody
TRAcP (Tartrate-Resistant Acid Phosphatase) (Hairy Cell Leukemia Marker)
Gene Names
ACP5; HPAP; TRAP; TRACP5a; TRACP5b; TrATPase
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Synonyms
TRAcP (Tartrate-Resistant Acid Phosphatase), Antibody; TRAcP (Tartrate-Resistant Acid Phosphatase) (Hairy Cell Leukemia Marker); Acid phosphatase 5, tartrate resistant; ACP5; serum band 5 tartrate-resistant acid phosphatase; SPENCDI; Tartrate-resistant acid ATPase; Tartrate-resistant acid phosphatase type 5; TR-AP; TRACP5; TRAcP; TRAP; TrATPase; Type 5 acid phosphatase; anti-TRAcP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Specificity
It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis. Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gauchers Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
325
Applicable Applications for anti-TRAcP antibody
IHC (Immunohistochemistry)
Immunogen
Recombinant full-length human ACP5 protein
Cellular Localization
Cytoplasmic (Lysosomes)
Hu-chromosome Location
19p13.2
Positive Control
HepG2, 293T, K562 or RPMI-8226 Cells. Spleen from Hairy Cell Leukemia (HCL) patient.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Non-hazardous. No MSDS required.
Non-hazardous. No MSDS required.
SDS-PAGE
(SDS-PAGE Analysis of Purified TRAcP Rabbit Recombinant Monoclonal Antibody (ACP5/2336R).)
SDS-PAGE
(SDS-PAGE Analysis of Purified TRAcP Rabbit Recombinant Monoclonal Antibody (ACP5/2336R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Spleen stained with TRAcP Rabbit Recombinant Monoclonal Antibody (ACP5/2336R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Spleen stained with TRAcP Rabbit Recombinant Monoclonal Antibody (ACP5/2336R).)
References
Capeller, B., Caffier, H., Sutterlin, M.W. and Dietl, J. 2003. Evaluation of tartrate-resistant acid phosphatase (TRAP) 5b as serum marker of bone metastases in human breast cancer. Anticancer Res. 23: 1011-1015.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35kDa
NCBI Official Full Name
tartrate-resistant acid phosphatase type 5
NCBI Official Synonym Full Names
acid phosphatase 5, tartrate resistant
NCBI Official Symbol
ACP5
NCBI Official Synonym Symbols
HPAP; TRAP; TRACP5a; TRACP5b; TrATPase
NCBI Protein Information
tartrate-resistant acid phosphatase type 5
UniProt Protein Name
Tartrate-resistant acid phosphatase type 5
UniProt Gene Name
ACP5
UniProt Synonym Gene Names
TR-AP; TrATPase
Similar Products
Product Notes
The TRAcP acp5 (Catalog #AAA214479) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRAcP (Tartrate-Resistant Acid Phosphatase) (Hairy Cell Leukemia Marker) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRAcP (Tartrate-Resistant Acid Phosphatase) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the TRAcP acp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAcP (Tartrate-Resistant Acid Phosphatase), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.