Mouse TRIM27 Monoclonal Antibody | anti-TRIM27 antibody

TRIM27 Mouse Monoclonal Antibody [Clone PCRP-TRIM27-1B3]

Cat. Num. AAA215892

• Gene Names: TRIM27; RFP; RNF76
• Reactivity: Human; Predicted to react with Mouse and Rat.
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: TRIM27, Antibody; TRIM27 Mouse Monoclonal Antibody [Clone PCRP-TRIM27-1B3]; AW538890; DAAP-182E11. 3; MGC189472; Ret finger protein; RFP transforming protein; Rfp1; RING finger protein 76; RNF76; Tripartite motif-containing protein 27; Zinc finger protein RFP; anti-TRIM27 antibody

• Host: Mouse
• Reactivity: Human; Predicted to react with Mouse and Rat.
• Clonality: Monoclonal
• Isotype: Mouse/IgG2a
• Clone Number: PCRP-TRIM27-1B3
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-TRIM27 antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant full-length human TRIM27 protein
• Cellular Localization: Nucleus, Cytoplasm, Nucleus > PML body, Nuclear or cytoplasmic depending on the cell type (By similarity), Colocalized with PML and EIF3S6 in nuclear bodies
• Positive Control: HeLa, U87 or Raji cells.
• Hu-chromosome Location: 6p22.1
• Unigene: 440382
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. TRIM27 Mouse Monoclonal Antibody (PCRP-TRIM27-1B3) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).)

Related Product Information for anti-TRIM27 antibody

Defects in TRIM27 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM27/RFP is found in thyroid papillary carcinomas. Translocation t(6;10)(p21. 3;q11. 2) with RET. The translocation generates TRIM27/RET and delta TRIM27/RET oncogenes.

NCBI and Uniprot Product Information

• NCBI GI #: 5730009
• NCBI GeneID: 5987
• NCBI Accession #: NP_006501
• NCBI GenBank Nucleotide #: NM_006510
• UniProt Accession #: P14373; Q5RJA8; Q5ST26; Q6LA73; Q6NXR9; Q9BZY6; Q9UJL3; A2BE15
• Molecular Weight: 41,439 Da
• NCBI Official Full Name: zinc finger protein RFP
• NCBI Official Synonym Full Names: tripartite motif containing 27
• NCBI Official Symbol: TRIM27
• NCBI Official Synonym Symbols: RFP; RNF76
• NCBI Protein Information: zinc finger protein RFP; RFP transforming protein; RING finger protein 76; ret finger protein; tripartite motif protein TRIM27; tripartite motif-containing 27; tripartite motif-containing protein 27
• UniProt Protein Name: Zinc finger protein RFP
• UniProt Gene Name: TRIM27
• UniProt Synonym Gene Names: RFP; RNF76
• UniProt Entry Name: TRI27_HUMAN