ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation was performed with 10 ug of cross-linked chromatin from HeLa, using 2 ug of TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) and Rabbit Control IgG (AC005). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.)
Rabbit anti-Human TriMethyl-Histone H3-K27 Monoclonal Antibody | anti-H3C1/H3-4 antibody
TriMethyl-Histone H3-K27 Rabbit PolymAb
DB: 1:500-1:1000
IHC-P: 1:50-1:200
IF/ICC: 1:50-1:200
ELISA: Recommended starting concentration is 1ug/mL.
Please optimize the concentration based on your specific assay requirements.
ChIP: 2 ug antibody for 5ug-10ug of Chromatin
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation was performed with 10 ug of cross-linked chromatin from HeLa, using 2 ug of TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) and Rabbit Control IgG (AC005). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation was performed with 10 ug of cross-linked chromatin from HeLa, using 2 ug of TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) and Rabbit Control IgG (AC005). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.)
ICC (Immunocytochemistry)
(Confocal imaging of C6 cells using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of C6 cells using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of HeLa cells using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.)
ICC (Immunocytochemistry)
(Confocal imaging of HeLa cells using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Human breast tissue using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Human breast tissue using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistchemistry)
(Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistchemistry)
(Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.)
WB (Western Blot)
(Western blot analysis of various lysates using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571)at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): H3 prokaryotic proteinExposure time: 5s.)
WB (Western Blot)
(Western blot analysis of various lysates using TriMethyl-Histone H3-K27 Rabbit PolymAb® (AAA28571)at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): H3 prokaryotic proteinExposure time: 5s.)
DB (Dot Blot)
(Dot-blot analysis of H3K9me0, H3K9me1, H3K9me2, H3K9me3, H3K14me0, H3K14me1, H3K14me2, H3K14me3, H3K27me0, H3K27me1, H3K27me2, H3K27me3?H3K36me0, H3K36me1, H3K36me2, H3K36me3?H3K79me0, H3K79me1, H3K79me2, H3K79me3 using TriMethyl-Histone H3-K27 Rabbit PolymAb® .)
DB (Dot Blot)
(Dot-blot analysis of H3K9me0, H3K9me1, H3K9me2, H3K9me3, H3K14me0, H3K14me1, H3K14me2, H3K14me3, H3K27me0, H3K27me1, H3K27me2, H3K27me3?H3K36me0, H3K36me1, H3K36me2, H3K36me3?H3K79me0, H3K79me1, H3K79me2, H3K79me3 using TriMethyl-Histone H3-K27 Rabbit PolymAb® .)
NCBI and Uniprot Product Information
Observed MW: 17kDa
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Product Notes
The H3C1/H3-4 hist3h3 (Catalog #AAA28571) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TriMethyl-Histone H3-K27 Rabbit PolymAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TriMethyl-Histone H3-K27 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Dot Blot (DB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Chromatin Immunoprecipitation (ChIP). WB: 1:500-1:1000 DB: 1:500-1:1000 IHC-P: 1:50-1:200 IF/ICC: 1:50-1:200 ELISA: Recommended starting concentration is 1ug/mL. Please optimize the concentration based on your specific assay requirements. ChIP: 2 ug antibody for 5ug-10ug of Chromatin. Researchers should empirically determine the suitability of the H3C1/H3-4 hist3h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEACEAY. It is sometimes possible for the material contained within the vial of "TriMethyl-Histone H3-K27, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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