Mouse anti-Human Tryptase Monoclonal Antibody | anti-TPSAB1 antibody

Tryptase (Mast Cell Marker)

Cat. Num. AAA215235

• Gene Names: TPSAB1; TPS1; TPS2; TPSB1; TPSB2; Tryptase-2
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Tryptase, Antibody; Tryptase (Mast Cell Marker); Lung Tryptase; Mast cell alpha II Tryptase; Mast cell beta I Tryptase; Mast cell protease 7; Mast cell protease II; MCP7; Pituitary Tryptase; Skin Tryptase; TPS1; TPSAB1; TPSB1; Tryptase 1; Tryptase alpha 1; Tryptase skin; anti-TPSAB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: TPSAB1/1963
• Specificity: Tryptases comprise a family of trypsin-like serine proteases (peptidase family S1). Tryptases are stored in mast cell secretory granules and basophils. Mast cells are connective tissue cells derived from blood-forming tissues that line arterial walls and secrete substances, which mediate inflammatory and immune responses. Tryptases are released into the extracellular environment and are resistant to all known endogenous proteinase inhibitors. This antibody reacts with mast cells distributed in skin, synovium, lung, and heart. This antibody does not bind with any other cell type. Human mast cell tryptase is considered to be an important marker of mast cell activation and is an important mediator of inflammation. Mastocytosis is a term collectively used for a group of disorders in which there is abnormal accumulation of mast cells in one or multiple organs. Anti-tryptase, combined with anti-CD2, anti-CD25, and anti-CD117, can be useful in identifying reactive mast cell hyperplasia, myelogenous neoplasms, mast cell leukemia, and mastocytosis.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 275

• Applicable Applications for anti-TPSAB1 antibody: IHC (Immunohistochemistry)
• Positive Control: Skin or Tonsil.
• Immunogen: Recombinant human Tryptase protein fragment (around aa 115-233) (Exact sequence is proprietary)
• Cellular Localization: Secreted and Cytoplasmic
• Hu-chromosome Location: 16p13.3
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified Tryptase Mouse Monoclonal Antibody (TPSAB1/1963). Confirmation of Integrity and Purity of Antibody.)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Tryptase Mouse Monoclonal Antibody (TPSAB1/1963).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Skin stained with Tryptase Mouse Monoclonal Antibody (TPSAB1/1963).)

References

Gurish, M.F. and Austen, K.F. 2001. The diverse roles of mast cells. J. Exp. Med. 194: F1-F5

NCBI and Uniprot Product Information

• NCBI GI #: 1182067
• NCBI GeneID: 7177
• UniProt Accession #: Q15661
• Molecular Weight: 31-36kDa
• NCBI Official Full Name: tryptase
• NCBI Official Synonym Full Names: tryptase alpha/beta 1
• NCBI Official Symbol: TPSAB1
• NCBI Official Synonym Symbols: TPS1; TPS2; TPSB1; TPSB2; Tryptase-2
• NCBI Protein Information: tryptase alpha/beta-1
• UniProt Protein Name: Tryptase alpha/beta-1
• UniProt Gene Name: TPSAB1
• UniProt Synonym Gene Names: TPS1; TPS2; TPSB1; Tryptase-1
• UniProt Entry Name: TRYB1_HUMAN