Standard Curve (Sample)
(Fig.1. Mouse Tryptase alpha/beta-1 (TPSAB1) Standard Curve.)
Mouse Tryptase alpha/beta-1 (TPSAB1) ELISA Kit | TPSAB1 elisa kit
Mouse Tryptase alpha/beta-1 (TPSAB1) ELISA Kit
Gene Names
Tpsab1; Mcp7; Mcp-7; Mcpt7; MMCP-7
Reactivity
Mouse
Synonyms
Tryptase alpha/beta-1 (TPSAB1); N/A; Mouse Tryptase alpha/beta-1 (TPSAB1) ELISA Kit; TPSAB1; TPS1; TPS2; TPSB1; mast cell alpha tryptase; mast cell beta I tryptase; tryptase alpha; tryptase beta 1; tryptase beta I; tryptase-I; tryptase-III; TPSAB1 elisa kit
Reactivity
Mouse
Specificity
Mouse Tryptase alpha/beta-1 (TPSAB1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TPSAB1. No significant cross-reactivity or interference between Mouse TPSAB1 and analogues was observed.
Sequence Length
273
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Tryptase alpha/beta-1 (TPSAB1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Tryptase alpha/beta-1 (TPSAB1) Standard Curve.)
Related Product Information for TPSAB1 elisa kit
Description: This Mouse Tryptase alpha/beta-1 (TPSAB1) ELISA Kit employs a two-site sandwich ELISA to quantitate TPSAB1 in samples. An antibody specific for TPSAB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPSAB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPSAB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPSAB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SLC16A1 mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT. The corresponding cDNA was isolated by an expression cloning strategy and found to encode a protein with 12 putative membrane-spanning regions. The cloned mutant 'mevalonate transporter' differed from its wildtype progenitor by 1 amino acid in the tenth membrane-spanning region, which changed a phenylalanine (wildtype) to a cysteine (mutant). The finding that the wildtype cDNA did not elicit increased mevalonate transport in transfected cells suggested that the wildtype protein is a transporter for a molecule other than mevalonate.
Background: SLC16A1 mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT. The corresponding cDNA was isolated by an expression cloning strategy and found to encode a protein with 12 putative membrane-spanning regions. The cloned mutant 'mevalonate transporter' differed from its wildtype progenitor by 1 amino acid in the tenth membrane-spanning region, which changed a phenylalanine (wildtype) to a cysteine (mutant). The finding that the wildtype cDNA did not elicit increased mevalonate transport in transfected cells suggested that the wildtype protein is a transporter for a molecule other than mevalonate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tryptase
NCBI Official Synonym Full Names
tryptase alpha/beta 1
NCBI Official Symbol
Tpsab1
NCBI Official Synonym Symbols
Mcp7; Mcp-7; Mcpt7; MMCP-7
NCBI Protein Information
tryptase
UniProt Protein Name
Tryptase
UniProt Gene Name
Tpsab1
UniProt Synonym Gene Names
Mcpt7; mMCP-7
UniProt Entry Name
TRYB1_MOUSE
Similar Products
Product Notes
The Mouse TPSAB1 tpsab1 (Catalog #AAA332077) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332077 ELISA Kit recognizes Mouse TPSAB1. It is sometimes possible for the material contained within the vial of "Tryptase alpha/beta-1 (TPSAB1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.