ELISA
(Sandwich ELISA analysis of human TSLP using Mouse anti Human TSLP (AAA50436) as a capture reagent and biotinylated Mouse anti Human TSLP as a detection reagent with purified recombinant TSLP as the antigen. Detection was performed using HRP co)
Mouse anti-Human TSLP Monoclonal Antibody | anti-TSLP antibody
Mouse anti Human TSLP
Reactivity
Human
Applications
ELISA
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
TSLP, Antibody; Mouse anti Human TSLP; TSLP antibody | H03-5E12; anti-TSLP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
H03-5E12
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
Purified IgG - liquid
0.09% Sodium Azide (NaN3)
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-TSLP antibody
ELISA
Immunogen
E Coli derived recombinant full length human TSLP (Tyr29-Gln159)
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
ELISA
(Sandwich ELISA analysis of human TSLP using Mouse anti Human TSLP (AAA50436) as a capture reagent and biotinylated Mouse anti Human TSLP as a detection reagent with purified recombinant TSLP as the antigen. Detection was performed using HRP co)
ELISA
(Sandwich ELISA analysis of human TSLP using Mouse anti Human TSLP (AAA50436) as a capture reagent and biotinylated Mouse anti Human TSLP as a detection reagent with purified recombinant TSLP as the antigen. Detection was performed using HRP co)
Related Product Information for anti-TSLP antibody
Mouse anti Human TSLP antibody, clone H03-5E12, recognizes thymic stromal lymphopoietin (TSLP), a member of the interleukin-2 (IL-2) cytokine family and an IL-7-like cytokine. TSLP plays a role in pathophysiological immunity-related functions. Specifically, TSLP is implicated in allergic reaction, infection, cancer and autoimmunity (Ziegler et al. 2013). During allergic inflammation, the primary producers of TSLP are epithelial cells, keratinocytes, mast cells, smooth muscle cells, dendritic cells and trophoblasts (Takai 2012).
TSLP is a ligand of the TSLP receptor (TSLPR), which is expressed by a variety of hematopoietic cell populations and some hematopoietic cells such as epithelial cells (Ziegler et al. 2013).Upon binding of TSLP to TSLPR various signaling pathways can be activated including JAK, STAT, Src kinases and PI3K. Under physiological conditions, induction of these pathways can promote homeostatic polyclonal expansion of T cells in the absence of foreign antigens and drive differentiation of inducible Tregs (Nakajima et al. 2020).
The purified Mouse anti Human TSLP antibody, clone H03-5E12 can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human TSLP antibody, clone D01-2E2 as the detection antibody.
TSLP is a ligand of the TSLP receptor (TSLPR), which is expressed by a variety of hematopoietic cell populations and some hematopoietic cells such as epithelial cells (Ziegler et al. 2013).Upon binding of TSLP to TSLPR various signaling pathways can be activated including JAK, STAT, Src kinases and PI3K. Under physiological conditions, induction of these pathways can promote homeostatic polyclonal expansion of T cells in the absence of foreign antigens and drive differentiation of inducible Tregs (Nakajima et al. 2020).
The purified Mouse anti Human TSLP antibody, clone H03-5E12 can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human TSLP antibody, clone D01-2E2 as the detection antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
thymic stromal lymphopoietin isoform 2
NCBI Official Synonym Full Names
thymic stromal lymphopoietin
NCBI Official Symbol
TSLP
NCBI Protein Information
thymic stromal lymphopoietin
UniProt Protein Name
Thymic stromal lymphopoietin
UniProt Gene Name
TSLP
UniProt Entry Name
TSLP_HUMAN
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Product Notes
The TSLP tslp (Catalog #AAA50436) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human TSLP reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TSLP can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the TSLP tslp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSLP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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