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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of N2A cells with Tubulin beta-III antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit Tubulin beta-III Monoclonal Antibody | anti-TUBB3 antibody
Tubulin beta-III Antibody
Gene Names
TUBB3; CDCBM; TUBB4; beta-4; CFEOM3A
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
Tubulin beta-III, Antibody; Tubulin beta-III Antibody; beta 3 tubulin antibody; beta-4 antibody; CDCBM antibody; CDCBM1 antibody; CFEOM3 antibody; CFEOM3A antibody; FEOM3 antibody; M (beta)3 antibody; M (beta)6 antibody; MC1R antibody; Neuron specific beta III Tubulin antibody; Neuron-specific class III beta-tubulin antibody; QccE-11995 antibody; QccE-15186 antibody; TBB3_HUMAN antibody; Tubb 3 antibody; TUBB3 antibody; TUBB4 antibody; Tubulin beta 3 antibody; Tubulin beta 3 chain antibody; Tubulin beta 4 antibody; Tubulin beta III antibody; Tubulin beta-3 chain antibody; Tubulin beta-4 chain antibody; Tubulin beta-III antibody; anti-TUBB3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SP06-00
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-TUBB3 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of N2A cells with Tubulin beta-III antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of N2A cells with Tubulin beta-III antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Tubulin beta-III in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Tubulin beta-III in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Tubulin beta-III in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Tubulin beta-III in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tubulin beta-III antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tubulin beta-III antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Tubulin beta-III on different lysates using anti-Tubulin beta-III antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12 Lane 3: SH-SY-5Y)
WB (Western Blot)
(Western blot analysis of Tubulin beta-III on different lysates using anti-Tubulin beta-III antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12 Lane 3: SH-SY-5Y)
Related Product Information for anti-TUBB3 antibody
Tubulin is a major cytoskeleton component that has five distinct forms, designated alpha, beta , gamma, delta and e Tubulin. alpha and beta Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple beta Tubulin isoforms (beta 1, beta 2, beta 3, beta 4, beta 5, beta 6 and beta 8) have been characterized and are expressed in mammalian tissues. beta 1 and beta 4 are present throughout the cytosol, beta 2 is present in the nuclei and nucleoplasm, and beta 3 is a neuron-specific cytoskeletal protein. gamma Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both delta Tubulin and e Tubulin are associated with the centrosome. delta Tubulin is a homolog of the Chlamydomonas delta Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.
Product Categories/Family for anti-TUBB3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,433 Da
NCBI Official Full Name
tubulin beta-3 chain isoform 2
NCBI Official Synonym Full Names
tubulin, beta 3 class III
NCBI Official Symbol
TUBB3
NCBI Official Synonym Symbols
CDCBM; TUBB4; beta-4; CFEOM3A
NCBI Protein Information
tubulin beta-3 chain; tubulin beta-III; tubulin beta-4 chain; class III beta-tubulin
UniProt Protein Name
Tubulin beta-3 chain
UniProt Gene Name
TUBB3
UniProt Synonym Gene Names
TUBB4
UniProt Entry Name
TBB3_HUMAN
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Product Notes
The TUBB3 tubb3 (Catalog #AAA312169) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Tubulin beta-III Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin beta-III can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TUBB3 tubb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin beta-III, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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