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ICC (Immunocytochemistry)
(ICC staining U1A in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit U1A Monoclonal Antibody | anti-U1A antibody
U1A Antibody
Gene Names
SNRPA; U1A; Mud1; U1-A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
U1A, Antibody; U1A Antibody; fc19d01 antibody; Mud1 antibody; small nuclear ribonucleoprotein polypeptide A antibody; snRNP A antibody; snRNP protein A antibody; SNRPA antibody; SNRPA_HUMAN antibody; U1 small nuclear ribonucleoprotein A antibody; U1 small nuclear RNP specific A antibody; U1 snRNP A antibody; U1 snRNP specific protein A antibody; U1-A antibody; U1A antibody; wu:fc19d01 antibody; zgc:77810 antibody; anti-U1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JB33-48
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-U1A antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:50-1:100
Immunogen
Recombinant protein corresponding to N-terminal human U1A.
Preparation and Storage
Store at -20°C.
ICC (Immunocytochemistry)
(ICC staining U1A in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining U1A in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining U1A in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining U1A in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining U1A in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining U1A in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-U1A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-U1A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-U1A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-U1A antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of U1A on different lysates using anti-U1A antibody at 1/500 dilution. Positive control: Lane 1: Rat brain Lane 2: K562 Lane 3: A431 Lane 4: Mouse kidney)
WB (Western Blot)
(Western blot analysis of U1A on different lysates using anti-U1A antibody at 1/500 dilution. Positive control: Lane 1: Rat brain Lane 2: K562 Lane 3: A431 Lane 4: Mouse kidney)
Related Product Information for anti-U1A antibody
SNRPA (small nuclear ribonucleoprotein polypeptide A), also known as U1A (U1 snRNP protein A), is a component of the RNA spliceosome, a complex of proteins that are required for the precise excision of introns from pre-messenger RNA (pre-mRNA). Localizing to the nucleus, SNRPA contains two RRM (RNA recognition motif) domains, namely RRM1 and RRM2, and RRM1 specifically associates with the stem loop II of U1 snRNA (small nuclear RNA). In addition to functioning as a component of the U1 snRNP, SNRPA negatively regulates polyadenylation of SNRPA pre-mRNA, thereby negatively regulating itself. By inhibiting the addition of a polyA tail that would allow the pre-mRNA to mature, SNRPA causes the nuclear exosome degradation of the SNRPA pre-mRNA. At least 16% of cellular SNRPA also functions in an snRNP-free form (SF-A) that complexes with a group of non-snRNP proteins.
Product Categories/Family for anti-U1A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31 kDa
NCBI Official Full Name
U1 small nuclear ribonucleoprotein A
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein polypeptide A
NCBI Official Symbol
SNRPA
NCBI Official Synonym Symbols
U1A; Mud1; U1-A
NCBI Protein Information
U1 small nuclear ribonucleoprotein A; U1 snRNP A; U1 snRNP-specific protein A; U1 small nuclear RNP-specific A
UniProt Protein Name
U1 small nuclear ribonucleoprotein A
UniProt Gene Name
SNRPA
UniProt Synonym Gene Names
U1 snRNP A; U1-A; U1A
UniProt Entry Name
SNRPA_HUMAN
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Product Notes
The U1A snrpa (Catalog #AAA30476) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The U1A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's U1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200 IP: 1:50-1:100. Researchers should empirically determine the suitability of the U1A snrpa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "U1A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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