Mouse UBE2B Monoclonal Antibody | anti-UBE2B antibody

UBE2B Mouse Monoclonal Antibody [Clone PCRP-UBE2B-1C7]

Cat. Num. AAA215973

• Gene Names: UBE2B; HR6B; UBC2; HHR6B; RAD6B; E2-17kDa
• Reactivity: Human; Predicted to work in Mouse, Rat, Xenopus and Zebrafish.
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: UBE2B, Antibody; UBE2B Mouse Monoclonal Antibody [Clone PCRP-UBE2B-1C7]; E2 17kDa; hHR6B; HR6B; RAD6 homolog B (RAD6B); UBC2; UBE2B; Ubiquitin carrier protein B; Ubiquitin conjugating enzyme E2B; Ubiquitin protein ligase B; Ubiquitin-conjugating enzyme E2 17kDa;; anti-UBE2B antibody

• Host: Mouse
• Reactivity: Human; Predicted to work in Mouse, Rat, Xenopus and Zebrafish.
• Clonality: Monoclonal
• Isotype: Mouse/IgG2b
• Clone Number: PCRP-UBE2B-1C7
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-UBE2B antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant full-length human UBE2B protein
• Cellular Localization: Cell membrane, Nucleus, In peripheral neurons, expressed both at the plasma membrane and in nuclei
• Positive Control: HeLa, K562 or Jurkat cells.
• Hu-chromosome Location: 5q31.1
• Unigene: 644421 & 612096
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. UBE2B Mouse Monoclonal Antibody (PCRP-UBE2B-1C7) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).)

Related Product Information for anti-UBE2B antibody

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (ubiquitin-conjugating enzyme E2 A) and UBE2B (ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to post replication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.

NCBI and Uniprot Product Information

• NCBI GI #: 52783814
• NCBI GeneID: 7320
• UniProt Accession #: P63146; P23567; Q4PJ15; Q9D0J6; B2R503; D3DQA2
• Molecular Weight: 152
• NCBI Official Full Name: Ubiquitin-conjugating enzyme E2 B
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2B
• NCBI Official Symbol: UBE2B
• NCBI Official Synonym Symbols: HR6B; UBC2; HHR6B; RAD6B; E2-17kDa
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 B; E2 protein; RAD6 homolog B; ubiquitin-protein ligase B; ubiquitin carrier protein B; ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog)
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 B
• UniProt Gene Name: UBE2B
• UniProt Synonym Gene Names: RAD6B; HR6B; hHR6B
• UniProt Entry Name: UBE2B_HUMAN