Application Data
(AMP-Glo assay for UBE2B activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2B in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
UBE2B recombinant protein
Recombinant UBE2B protein
Purity
>=95% by SDS-PAGE.
Synonyms
UBE2B; N/A; Recombinant UBE2B protein; Ubiquitin conjugating enzyme E2 B; E2-17kDa; HHR6B; RAD6B; UBC2; UBE2B recombinant protein
Host
E coli
Purity/Purification
>=95% by SDS-PAGE.
Form/Format
Recombinant UBE2B protein is supplied in 25 mM Tris 8.0, 300mM NaCl, 20% glycerol, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant UBE2B protein that includes full length of human UBE2B protein (accession number NP_003328.1) was expressed in E coli and contains an N-terminal His tag with a molecular weight of 19.48kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(AMP-Glo assay for UBE2B activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2B in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
Application Data
(AMP-Glo assay for UBE2B activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2B in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
SDS-PAGE
(Recombinant UBE2B protein gel. UBE2B protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 19.48kDa Purity: >95%)
SDS-PAGE
(Recombinant UBE2B protein gel. UBE2B protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 19.48kDa Purity: >95%)
Related Product Information for UBE2B recombinant protein
UBE2B (Ubiquitin-conjugating enzyme E2 B) also known as E2-17kDa, HHR6B, HR6B, RAD6B and UBC2. UBE2B is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2B can accept ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at ‘Lys-120’ to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes ‘Lys-11’-, as well as ‘Lys-48’- and ‘Lys-63’-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on ‘Lys-164’. May be involved in neurite outgrowth.
Product Categories/Family for UBE2B recombinant protein
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Product Notes
The UBE2B (Catalog #AAA60400) is a Recombinant Protein produced from E coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "UBE2B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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