Mouse anti-Human Ubiquitin-conjugating Enzyme E2C/UBCH10 Monoclonal Antibody | anti-UBE2C antibody

Ubiquitin-conjugating Enzyme E2C/UBCH10

Cat. Num. AAA215376

• Gene Names: UBE2C; UBCH10; dJ447F3.2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Ubiquitin-conjugating Enzyme E2C/UBCH10, Antibody; Ubiquitin-conjugating Enzyme E2C/UBCH10; Cyclin selective ubiquitin carrier protein; mitotic specific ubiquitin conjugating enzyme; UB E2C; UBCH10; Ubiquitin protein ligase C; anti-UBE2C antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: CPTC-UBE2C-1
• Specificity: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ubconjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2C, also designated UBCH10 in human, is an E2 ubiquitin conjugating enzyme for the anaphase promoting complex (APC), which coordinates mitosis and G1 by sequentially promoting the degradation of key cell-cycle regulators. UBE2C is overexpressed in many different types of cancers and may be a potential therapeutic target.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 132

• Applicable Applications for anti-UBE2C antibody: IHC (Immunohistochemistry), ELISA
• Immunogen: Recombinant Human full-length UBE2C protein
• Cellular Localization: Cytoplasm
• Hu-chromosome Location: 20q13.12
• Positive Control: HeLa, Jurkat or A431 cells. Human Tonsil or Placenta tissue.
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified UBE2C Mouse Monoclonal Antibody (CPTC-UBE2C-1). Confirmation of Integrity and Purity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human brain stained with UBE2C Mouse Monoclonal Antibody (CPTC-UBE2C-1))

References

Lin, Y., et al. 2002. Structural and functional analysis of the human mitoticspecific ubiquitin-conjugating enzyme, UBCH10. J. Biol. Chem. 277: 21913-21921.

NCBI and Uniprot Product Information

• NCBI GI #: 528524529
• NCBI GeneID: 11065
• NCBI Accession #: NP_001268670.1
• NCBI GenBank Nucleotide #: NP_001268670.1
• UniProt Accession #: O00762
• Molecular Weight: 20kDa
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 C isoform 6
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 C
• NCBI Official Symbol: UBE2C
• NCBI Official Synonym Symbols: UBCH10; dJ447F3.2
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 C
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 C
• UniProt Gene Name: UBE2C
• UniProt Synonym Gene Names: UBCH10
• UniProt Entry Name: UBE2C_HUMAN