Rabbit Ubiquitin Polyclonal Antibody | anti-Ubiquitin antibody

Ubiquitin Polyclonal Antibody

Cat. Num. AAA179537

• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity purification
• Synonyms: Ubiquitin, Antibody; Ubiquitin Polyclonal Antibody; UBB; HEL-S-50; ubiquitin B; anti-Ubiquitin antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-Ubiquitin antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant fusion protein of human Ubiquitin
• Research Area: Cell Biology
• Preparation and Storage: Storage: -20 degree C/One year. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cells using Ubiquitin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of PC-12 cells using Ubiquitin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using Ubiquitin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human esophageal cancer using Ubiquitin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines using Ubiquitin Polyclonal Antibody at 1:1000 dilution.NIH/3T3 cells were treated by MG132(50 uM) at 37? for 90 minutes.)

Related Product Information for anti-Ubiquitin antibody

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-Ubiquitin antibody

Antibodies; Primary Antibodies; Polyclonal Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 302595875
• NCBI GeneID: 7314
• UniProt Accession #: P0CG47; P02248; P02249; P02250; P62988; Q29120; Q6LBL4; Q6LDU5; Q8WYN8; Q91887; Q91888; Q9BWD6
• Molecular Weight: 229
• NCBI Official Full Name: Polyubiquitin-B
• NCBI Official Synonym Full Names: ubiquitin B
• NCBI Official Symbol: UBB
• NCBI Protein Information: polyubiquitin-B; polyubiquitin B
• UniProt Protein Name: Polyubiquitin-B
• UniProt Gene Name: UBB
• UniProt Entry Name: UBB_HUMAN